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A low cost remote imaging platform for biological applications was developed. The "Picroscope" is a device that allows the user to perform longitudinal imaging studies on multi-well cell culture plates. Here we present the network…

A metasurface lens (meta-lens) bends light using nanostructures on a flat surface. Macroscopic meta-lenses (mm- to cm-scale diameter) have been quite difficult to simulate and optimize, due to the large area, the lack of periodicity, and…

Optics · Physics 2016-03-04 Steven J. Byrnes , Alan Lenef , Francesco Aieta , Federico Capasso

Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…

For over three decades, scanning probe microscopy (SPM) has been a key method for exploring material structures and functionalities at nanometer and often atomic scales in ambient, liquid, and vacuum environments. Historically, SPM…

Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement make this method being state-of-the-art. This raises a question what maximal…

Image and Video Processing · Electrical Eng. & Systems 2020-10-29 Kirill Lonhus , Renata Rychtarikova , Ganna Platonova , Dalibor Stys

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovered unicellular organisms. Countless innovations have positioned light microscopy as a cornerstone of modern biology and a method…

Optical microscopy is an indispensable tool in life sciences research, but conventional techniques require compromises between imaging parameters like speed, resolution, field-of-view, and phototoxicity. To overcome these limitations,…

Quantitative Methods · Quantitative Biology 2024-04-01 Leonor Morgado , Estibaliz Gómez-de-Mariscal , Hannah S. Heil , Ricardo Henriques

Though quite challenging, leveraging large-scale unlabeled or partially labeled images in a cost-effective way has increasingly attracted interests for its great importance to computer vision. To tackle this problem, many Active Learning…

Computer Vision and Pattern Recognition · Computer Science 2018-05-25 Keze Wang , Xiaopeng Yan , Dongyu Zhang , Lei Zhang , Liang Lin

The use of electron mirrors in aberration correction and surface-sensitive microscopy techniques such as low-energy electron microscopy has been established. However, in this work, by implementing an easy to construct, fully electrostatic…

Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…

A simple, yet general, formalism for the optimized linear combination of astrophysical images is constructed and demonstrated. The formalism allows the user to combine multiple undersampled images to provide oversampled output at high…

Instrumentation and Methods for Astrophysics · Physics 2015-05-28 Barnaby Rowe , Christopher Hirata , Jason Rhodes

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by…

Quantitative Methods · Quantitative Biology 2023-08-09 Afonso Mendes , Hannah S. Heil , Simao Coelho , Christophe Leterrier , Ricardo Henriques

Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that…

Biological Physics · Physics 2019-11-06 Jiaming Guo , Camille Artur , Jason L. Eriksen , David Mayerich

Despite decades of research, the ultimate goal of nanotechnology--top-down manipulation of individual atoms--has been directly achieved with only one technique: scanning probe microscopy. In this Review, we demonstrate that scanning…

An automatic approach to counting any kind of cells could alleviate work of the experts and boost the research in fields such as regenerative medicine. In this paper, a method for microscopy cell counting using multiple frames (hence…

Computer Vision and Pattern Recognition · Computer Science 2018-02-01 Alexander Gomez Villa , Augusto Salazar , Igor Stefanini

Scanning superconducting quantum interference device microscopy (SSM) is a scanning probe technique that images local magnetic flux, which allows for mapping of magnetic fields with high field and spatial accuracy. Many studies involving…

Instrumentation and Detectors · Physics 2018-01-17 P. Reith , X. Renshaw Wang , H. Hilgenkamp

This paper proposes LLaFS, the first attempt to leverage large language models (LLMs) in few-shot segmentation. In contrast to the conventional few-shot segmentation methods that only rely on the limited and biased information from the…

Computer Vision and Pattern Recognition · Computer Science 2024-04-04 Lanyun Zhu , Tianrun Chen , Deyi Ji , Jieping Ye , Jun Liu

Many problems of low-level computer vision and image processing, such as denoising, deconvolution, tomographic reconstruction or super-resolution, can be addressed by maximizing the posterior distribution of a sparse linear model (SLM). We…

Machine Learning · Statistics 2010-08-16 Matthias W. Seeger , Hannes Nickisch