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Point spread function (PSF) engineering of the emitter can code higher spatial frequency information of an image to break diffraction limit but suffer from the complexed optical systems. Here we present a robust strategy to simultaneously…

Optics · Physics 2021-02-23 Chaohao Chen , Baolei Liu , Yongtao Liu , Jiayan Liao , Xuchen Shan , Fan Wang , Dayong Jin

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

Quantitative Methods · Quantitative Biology 2025-11-03 Surajit Das , Pavel Zun

Single-molecule sensitive microscopies and spectroscopies are transforming biophysics and materials science laboratories. Techniques such as fluorescence correlation spectroscopy (FCS) and single-molecule sensitive fluorescence resonance…

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

Computer Vision and Pattern Recognition · Computer Science 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

Classical sensors for spectrum analysis are widely used but lack micro- or nanoscale spatial resolution. On the other hand, quantum sensors, capable of working with nanoscale precision, do not provide precise frequency resolution over a…

Quantum Physics · Physics 2022-07-12 Nicolas Staudenmaier , Simon Schmitt , Liam P. McGuinness , Fedor Jelezko

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Determination of fundamental mechanisms of disease often hinges on histopathology visualization and quantitative image analysis. Currently, the analysis of multi-channel fluorescence tissue images is primarily achieved by manual…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Medical Hyperspectral Imaging (MHSI) has emerged as a promising tool for enhanced disease diagnosis, particularly in computational pathology, offering rich spectral information that aids in identifying subtle biochemical properties of…

Computer Vision and Pattern Recognition · Computer Science 2025-07-10 Qing Zhang , Guoquan Pei , Yan Wang

The advent of large instantaneous bandwidth receivers and high spectral resolution spectrometers on (sub-)millimeter telescopes has opened up the possibilities for unbiased spectral surveys. Because of the large amount of data they contain,…

Instrumentation and Methods for Astrophysics · Physics 2015-05-20 S. Maret , P. Hily-Blant , J. Pety , S. Bardeau , E. Reynier

We present a fully modular and scalable software pipeline for processing electron microscope (EM) images of brain slices into 3D visualization of individual neurons and demonstrate an end-to-end segmentation of a large EM volume using a…

Distributed, Parallel, and Cluster Computing · Computer Science 2020-11-20 Rafael Vescovi , Hanyu Li , Jeffery Kinnison , Murat Keceli , Misha Salim , Narayanan Kasthuri , Thomas D. Uram , Nicola Ferrier

We present a widefield two-dimensional electronic spectroscopy microscope (2DESM) that integrates multidimensional coherent spectroscopy with optical imaging, enabling femtosecond temporal and micrometer spatial resolution. The broadband…

Dynamic Magnetic Resonance Imaging (dMRI) is widely used to assess various cardiac conditions such as cardiac motion and blood flow. To accelerate MR acquisition, techniques such as undersampling and Simultaneous Multi-Slice (SMS) are often…

Image and Video Processing · Electrical Eng. & Systems 2023-01-05 Daniel H. Pak , Xiao Chen , Eric Z. Chen , Yikang Liu , Terrence Chen , Shanhui Sun

Real time computer control is an essential feature of scanning probe microscopes, which have become essential tools for the characterization and investigation of nanometer scale samples. Most commercial (and some open-source) scanning probe…

Mesoscale and Nanoscale Physics · Physics 2013-01-24 Venkat Chandrasekhar , Manan Mehta

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

We present a general procedure to decompose Beyond the Standard Model (BSM) collider signatures presenting a Z2 symmetry into Simplified Model Spectrum (SMS) topologies. Our method provides a way to cast BSM predictions for the LHC in a…

High Energy Physics - Phenomenology · Physics 2014-06-19 Sabine Kraml , Suchita Kulkarni , Ursula Laa , Andre Lessa , Wolfgang Magerl , Doris Proschofsky-Spindler , Wolfgang Waltenberger

In modern-day astronomy, near-infrared, optical, and ultraviolet spectroscopy are indispensable for studying a wide range of phenomena, from measuring black hole masses to analyzing chemical abundances in stellar atmospheres. However,…

Instrumentation and Methods for Astrophysics · Physics 2025-07-21 Raniere de Menezes , Francesco Massaro , Michela Negro , Claudia M. Raiteri , Harold Peña-Herazo , Jose A. Acosta-Pulido

Ultra-light dark matter candidates, such as axions and dark photons, particularly within the mass range of $\mu$eV to meV, have garnered significant research interest. However, the effectiveness of existing experiments is often hindered by…

Instrumentation and Detectors · Physics 2025-01-22 Hiroki Takeuchi , Junya Suzuki , Shunsuke Adachi , Osamu Tajima

Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…

Optics · Physics 2024-12-19 Rui Ma , Olga Lyraki , Daniel Wehner , Jochen Guck

High-spatial-resolution vibrational spectroscopy is one of the principal techniques for nanoscale compositional analysis in biological materials. Here, we present a new method for the analysis of whole-cell biological specimens through…

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