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Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by…

Biological Physics · Physics 2020-01-08 Courtney Johnson , Jack Exell , Jonathon Kuo , Kevin Welsher

Supervised deep learning approaches can artificially increase the resolution of microscopy images by learning a mapping between two image resolutions or modalities. However, such methods often require a large set of hard-to-get…

Image and Video Processing · Electrical Eng. & Systems 2024-11-20 Marzieh Gheisari , Auguste Genovesio

Super-resolution tasks oriented to images captured in ultra-dark environments is a practical yet challenging problem that has received little attention. Due to uneven illumination and low signal-to-noise ratio in dark environments, a…

Computer Vision and Pattern Recognition · Computer Science 2023-09-12 Jiaxin Gao , Ziyu Yue , Yaohua Liu , Sihan Xie , Xin Fan , Risheng Liu

Diffusion-weighted magnetic resonance imaging (DW-MRI) can be used to characterise the microstructure of the nervous tissue, e.g. to delineate brain white matter connections in a non-invasive manner via fibre tracking. Magnetic Resonance…

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…

Biological Physics · Physics 2018-08-10 Chiara Garbellotto , Jonathan M. Taylor

Microscope mode imaging for secondary ion mass spectrometry is a technique with the promise of simultaneous high spatial resolution and high speed imaging of biomolecules from complex surfaces. Technological developments such as new…

Instrumentation and Detectors · Physics 2013-09-05 András Kiss , Donald F. Smith , Julia H. Jungmann , Ron M. A. Heeren

Magnetic particle imaging (MPI) offers exceptional contrast for magnetic nanoparticles (MNP) at high spatio-temporal resolution. A common procedure in MPI starts with a calibration scan to measure the system matrix (SM), which is then used…

Image and Video Processing · Electrical Eng. & Systems 2022-11-04 Alper Güngör , Baris Askin , Damla Alptekin Soydan , Emine Ulku Saritas , Can Barış Top , Tolga Çukur

Despite super-resolution fluorescence blinking microscopes break the diffraction limit, the intense phototoxic illumination and long-term image sequences thus far still pose to major challenges in visualizing live-organisms. Here, we…

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

Super-resolved far-field microscopy has emerged as a powerful tool for investigating the structure of objects with resolution well below the diffraction limit of light. Nearly all super-resolution imaging techniques reported to date rely on…

Coherent interconversion of signals between optical and mechanical domains is enabled by optomechanical interactions. Extreme light-matter coupling produced by confining light to nanoscale mode volumes can then access single mid-infrared…

Structured illumination microscopy (SIM) is a wide-field super-resolution technique normally limited to roughly twice the diffraction-limited resolution ($\approx 100$--$200$~nm). Surpassing this bound is a classic ill-posed inverse…

Multi-image super-resolution (MISR) allows to increase the spatial resolution of a low-resolution (LR) acquisition by combining multiple images carrying complementary information in the form of sub-pixel offsets in the scene sampling, and…

Computer Vision and Pattern Recognition · Computer Science 2024-01-31 Luca Savant Aira , Diego Valsesia , Andrea Bordone Molini , Giulia Fracastoro , Enrico Magli , Andrea Mirabile

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multi-color capability with high-quality illumination is currently lacking.…

Optics · Physics 2023-03-14 Ning Xu , Sarah E. Bohndiek , Zexing Li , Cilong Zhang , Qiaofeng Tan

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

Optics · Physics 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

In wide-field nonlinear microscopy, wavefront modulation by means of phase-only spatial light modulators (SLMs) allows achieving simultaneous two-photon excitation and fluorescence emission from specific region-of-interests (ROIs) of…

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