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We introduce the white light quantitative phase imaging unit (WQPIU) as a practical realization of quantitative phase imaging (QPI) on standard microscope platforms. The WQPIU is a compact stand-alone unit which measures sample induced…

Optics · Physics 2016-05-04 YoonSeok Baek , KyeoReh Lee , Jonghee Yoon , Kyoohyun Kim , YongKeun Park

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Quantitative phase imaging (QPI) through multi-core fibers (MCFs) has been an emerging in vivo label-free endoscopic imaging modality with minimal invasiveness. However, the computational demands of conventional iterative phase retrieval…

Complex-field imaging is indispensable for numerous applications at wavelengths from X-ray to THz, with amplitude describing transmittance (or reflectivity) and phase revealing intrinsic structure of the target object. Coherent diffraction…

Image and Video Processing · Electrical Eng. & Systems 2021-04-07 Meng Li , Liheng Bian , Guoan Zheng , Andrew Maiden , Yang Liu , Yiming Li , Qionghai Dai , Jun Zhang

We introduce a single-frame diffractive imaging method called randomized probe imaging (RPI). In RPI, a sample is illuminated by a structured probe field containing speckles smaller than the sample's typical feature size. Quantitative…

Coherent diffractive imaging (CDI) provides new opportunities for high resolution X-ray imaging with simultaneous amplitude and phase contrast. Extensions to CDI broaden the scope of the technique for use in a wide variety of experimental…

Optics · Physics 2016-01-20 Matthew H. Seaberg , Alexandre d'Aspremont , Joshua J. Turner

Optical microscopy has been employed to derive salient characteristics of an object in various fields, including cell biology, flow cytometry, biopsy, and neuroscience. In particular, measuring the phase of light scattered from an object…

Optics · Physics 2025-03-18 Jeonghun Oh , Herve Hugonnet , Weisun Park , YongKeun Park

Label-free imaging approaches seek to simplify and augment histopathologic assessment by replacing the current practice of staining by dyes to visualize tissue morphology with quantitative optical measurements. Quantitative phase imaging…

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

As artificial intelligence becomes increasingly prevalent, the demand for faster and more energy-efficient computing approaches grows. While optical computing offers intrinsic advantages in bandwidth and power consumption, existing…

We present an algorithm for coherent diffractive imaging with phaseless measurements. It treats the forward model as a combination of coherent and incoherent waves. The algorithm reconstructs absorption and phase contrast that quantifies…

Computational Physics · Physics 2022-08-25 Miguel Moscoso , Alexei Novikov , George Papanicolaou , Chrysoula Tsogka

The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…

Optics · Physics 2020-01-08 Samira Ebrahimi , Masoomeh Dashtdar

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…

Optics · Physics 2022-04-20 Herve Hugonnet , Mahn Jae Lee , Yongkeun Park

High-throughput 3D quantitative phase imaging (QPI) in flow cytometry enables label-free, volumetric characterization of individual cells by reconstructing their refractive index (RI) distributions from multiple viewing angles during flow…

Computer Vision and Pattern Recognition · Computer Science 2025-09-08 Enze Ye , Wei Lin , Shaochi Ren , Yakun Liu , Xiaoping Li , Hao Wang , He Sun , Feng Pan

Coherent Diffractive Imaging is a lensless technique that allows imaging of matter at a spatial resolution not limited by lens aberrations. This technique exploits the measured diffraction pattern of a coherent beam scattered by periodic…

Computational Physics · Physics 2025-08-27 Alessandro Colombo , Davide Emilio Galli , Liberato De Caro , Francesco Scattarella , Elvio Carlino

Detection of phase variations across optically transparent samples is often a difficult task. We propose and demonstrate a compact, lightweight and low cost quantitative phase contrast imager. Light diffracted from a pinhole is incident on…

A single intensity-only holographic interferogram can records the full amplitude and phase information of optical field. However, current digital holography technologies cannot recover the lossless phase information from a single…

Image and Video Processing · Electrical Eng. & Systems 2020-03-09 Qinnan Zhang , Shengyu Lu , Jiaosheng Li , Wenjie Li , Dong Li , Xiaoxu Lu , Liyun Zhong , Jindong Tian

Label-free quantitative phase imaging is a vital tool for optical microscopy and metrology applications. A hair-thin multimode fiber stands out as a very attractive platform for minimally invasive imaging. Here we propose and experimentally…

Multi-spectral quantitative phase imaging (QPI) is an emerging imaging modality for wavelength dependent studies of several biological and industrial specimens. Simultaneous multi-spectral QPI is generally performed with color CCD cameras.…

Instrumentation and Detectors · Physics 2019-02-20 Azeem Ahmad , Anand Kumar , Vishesh Dubey , Ankit Butola , Balpreet Singh Ahluwalia , Dalip Singh Mehta

Despite offering high sensitivity, a high signal-to-noise ratio, and a broad spectral range, single-pixel imaging (SPI) is limited by low measurement efficiency and long data-acquisition times. To address this, we propose a…