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Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Next-generation particle accelerators demand advanced beam-diagnostic capabilities to ensure high performance, operational reliability, and sustainable machine operation. Increasing beam intensities and stored energies make the precise…

Accelerator Physics · Physics 2026-03-10 Francis René Osswald , Mohammed Chahbaoui , Xinyi Liang

With the advent of microsphere assisted microscopy in 2011, this technique emerged as a simple and easy way to obtain optical super-resolution. Although the possible mechanisms of imaging by microspheres are debated in the literature, most…

Optics · Physics 2015-08-27 Navid Farahi

Quantum imaging with undetected light has recently emerged as a technique in which quantum correlations and nonlinear interferometry are combined to decouple illumination and detection paths. This approach has been more recently extended…

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…

In recent years nanoscale coherent imaging has emerged as an indispensable imaging modality allowing to surpass the resolution limit given by classical imaging optics. At the same time, attosecond science has experienced enormous progress…

Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of…

Optics · Physics 2020-11-04 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Dielectric metasurfaces provide a unique platform for efficient harmonic generation and optical wavefront manipulation at the nanoscale. Tailoring phase and amplitude of a nonlinearly generated wave with a high emission efficiency using…

Optics · Physics 2022-10-03 David Hähnel , Jens Förstner , Viktor Myroshnychenko

We propose the use of the subwavelength localization via adiabatic passage technique for fluorescence microscopy with nanoscale resolution in the far field. This technique uses a {\Lambda}-type medium coherently coupled to two laser pulses:…

Quantum Physics · Physics 2015-04-14 Juan Luis Rubio , Daniel Viscor , Verònica Ahufinger , Jordi Mompart

Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining…

Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation…

Quantum simulations with ultracold atoms typically create atomic wavefunctions with structures at optical length scales, where direct imaging suffers from the diffraction limit. In analogy to advances in optical microscopy for biological…

Quantum Gases · Physics 2019-04-10 Sarthak Subhankar , Yang Wang , Tsz-Chun Tsui , Steven L. Rolston , James V. Porto

Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives…

Optics · Physics 2009-11-13 Stanislas Vertu , Jean-Jacques Delaunay , Olivier Haeberle

In recent years, a great deal of emphasis has been placed on achieving the diffraction limit with large aperture telescopes. For a well matched focal-plane instrument, the diffraction limit provides the highest possible angular resolution…

Astrophysics · Physics 2009-11-11 J. Bland-Hawthorn , A. Horton

We derive the fundamental limit to the resolution of far-field optical imaging, and demonstrate that, while a bound to the resolution of a fundamental nature does exit, contrary to the conventional wisdom it is neither exactly equal to nor…

Optics · Physics 2019-03-14 Evgenii Narimanov

The ability to perform broadband optical spectroscopy with sub-diffraction-limit resolution is highly sought-after for a wide range of critical applications. However, sophisticated tip-enhanced techniques are currently required to achieve…

First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence…

Optics · Physics 2023-08-02 Yilun Li , Fang Huang

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres.…

Optics · Physics 2013-12-25 Leonid A. Krivitsky , Jia Jun Wang , Zengbo Wang , Boris Lukiyanchuk

We numerically demonstrate inhibition of absorption, optical transparency, and anomalous momentum states of phase locked harmonic pulses in semiconductors, at UV and extreme UV frequencies, in spectral regions where the dielectric constant…

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