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The interaction between free electrons and optical near fields is attracting increasing attention as a way to manipulate the electron wave function in space, time, and energy. Relying on currently attainable experimental capabilities, we…

Optics · Physics 2020-07-22 Andrea Konečná , F. Javier García de Abajo

This publication presents an investigation of the performance of different analytical electron ptychography methods for low-dose imaging. In particular, benchmarking is performed for two model-objects, monolayer MoS$_2$ and apoferritin, by…

Applied Physics · Physics 2025-05-20 Hoelen L. Lalandec Robert , Max Leo Leidl , Knut Müller-Caspary , Jo Verbeeck

Here a new microscopic method is proposed to image and characterize very thin samples like few-layer materials, organic molecules, and nanostructures with nanometer or sub-nanometer resolution using electron beams of energies lower than 20…

Instrumentation and Detectors · Physics 2016-01-06 Ing-Shouh Hwang

Ptychography provides high dose efficiency images that can reveal light elements next to heavy atoms. However, despite ptychography having an otherwise single signed contrast transfer function, contrast reversals can occur when the…

Applied Physics · Physics 2023-06-16 C. Gao , C. Hofer , T. J. Pennycook

The appearance of direct electron detectors marked a new era for electron diffraction. Their high sensitivity and low noise opens the possibility to extend electron diffraction from transmission electron microscopes (TEM) to lower energies…

Applied Physics · Physics 2025-02-18 Nikita Denisov , Andrey Orekhov , Johan Verbeeck

X-ray phase contrast imaging offers a way to visualize the internal structures of an object without the need to deposit any radiation, and thereby alleviate the main concern in x-ray diagnostic imaging procedures today. Grating-based…

The vortex coronagraph is an optical instrument that precisely removes on-axis starlight allowing for high contrast imaging at small angular separation from the star, thereby providing a crucial capability for direct detection and…

Instrumentation and Methods for Astrophysics · Physics 2015-11-04 Garreth J. Ruane , Elsa Huby , Olivier Absil , Dimitri Mawet , Christian Delacroix , Brunella Carlomagno , Grover A. Swartzlander

Optical microscopy is without a doubt an essential component of life science research, but many objects of interest in biology are transparent. Chemical or immunological dyes, which can often be toxic, fluorescent transgenes, which require…

Optics · Physics 2012-09-11 Quintin Tyrell Davis

Quantum materials exhibit phases such as superconductivity at low temperatures, yet imaging their phase transition dynamics with high spatial resolution remains challenging due to conventional tools' limitations - scanning tunneling…

Mesoscale and Nanoscale Physics · Physics 2025-03-04 Chen Wang , Shaoshan Wang , Chuan Guo , Chengjian Yu , Qi Fu , Xiaopeng Xie , Changxi Zheng

The transmission electron microscope (TEM) has become an essential tool for innovation in nanoscience, material science, and biology. Despite these instruments being widely used across both industry and academia, academics may hesitate to…

Instrumentation and Detectors · Physics 2022-12-07 Patrick McBean , Zachary Milne , Arjun Kanthawar , Cameron O'Byrne , Khalid Hattar , Lewys Jones

Forescatter electron imaging is a popular microscopy technique, especially for scanning electron microscopes equipped with an electron backscatter diffraction detector. In principal, this method enables qualitative imaging of microstructure…

Materials Science · Physics 2018-06-04 Ben Britton , Daniel Goran , Vivian Tong

Next-generation cryogenic transmission electron microscopes (TEM) aim to achieve high-resolution imaging at ultracold sample temperatures (< 90 K) and over extended hold times. Lower temperatures enable atomic-scale characterization with…

The rigid-intensity-shift model of differential phase contrast scanning transmission electron microscopy (DPC-STEM) imaging assumes that the phase gradient imposed on the probe by the sample causes the diffraction pattern intensity to shift…

Instrumentation and Detectors · Physics 2018-04-25 L. Clark , H. G. Brown , D. M. Paganin , M. J. Morgan , T. Matsumoto , N. Shibata , T. C. Petersen , S. D. Findlay

Cryo-electron microscopy (cryo-EM) is capable of producing reconstructed 3D images of biomolecules at near-atomic resolution. As such, it represents one of the most promising imaging techniques in structural biology. However, raw cryo-EM…

Quantitative Methods · Quantitative Biology 2021-05-25 Nina Miolane , Frédéric Poitevin , Yee-Ting Li , Susan Holmes

The use of electron mirrors in aberration correction and surface-sensitive microscopy techniques such as low-energy electron microscopy has been established. However, in this work, by implementing an easy to construct, fully electrostatic…

Nowadays, modern electron microscopes deliver images at atomic scale. The precise atomic structure encodes information about material properties. Thus, an important ingredient in the image analysis is to locate the centers of the atoms…

Computer Vision and Pattern Recognition · Computer Science 2017-09-13 Benjamin Berkels , Benedikt Wirth

Optical methods for monitoring the electrochemical reaction at the interface are advantageous because of their table-top setup and ease of integration into reactors. Here we apply EDL-modulation microscopy to one of the main components of…

Chemical Physics · Physics 2023-03-21 Zhu Zhang , Sanli Faez

Cryo-electron microscopy (cryo-EM) has recently become a premier method for obtaining high-resolution structures of biological macromolecules. However, it is limited to biomolecular samples with low conformational heterogeneity, where all…

Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has…

Optics · Physics 2015-03-13 Tim N. Ford , Kengyeh K. Chu , Jerome Mertz

Single-particle cryo-electron microscopy (cryo-EM) reconstructs the three-dimensional (3D) structure of bio-molecules from a large set of 2D projection images with random and unknown orientations. A crucial step in the single-particle…

Image and Video Processing · Electrical Eng. & Systems 2020-06-24 Mona Zehni , Laurène Donati , Emmanuel Soubies , Zhizhen J. Zhao , Michael Unser
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