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In differential phase contrast scanning transmission electron microscopy (DPC-STEM), variability in dynamical diffraction resulting from changes in sample thickness and local crystal orientation (due to sample bending) can produce contrast…

Materials Science · Physics 2020-02-06 T. Mawson , A. Nakamura , T. C. Petersen , N. Shibata , H. Sasaki , D. M. Paganin , M. J. Morgan , S. D. Findlay

Scanning Electron Microscopy (SEM) is a widely used tool for nanoparticle characterization, but long-term directional drift can compromise image quality. We present a novel algorithm for post-imaging drift correction in SEM nanoparticle…

Instrumentation developments in electron energy-loss spectroscopy (EELS) in the scanning transmission electron microscope (STEM) one decade ago paved the way for combining milli-electronvolt energy resolution in spectroscopy with…

Mesoscale and Nanoscale Physics · Physics 2024-10-15 Benedikt Haas , Christoph T. Koch , Peter Rez

There is a clear need for developments in characterisation techniques that provide detailed information about structure-function relationships in biology. Using electron microscopy to achieve high resolution while maintaining a broad field…

Quantitative Methods · Quantitative Biology 2023-09-12 Chen Huang , Judy S. Kim , Angus I. Kirkland

Laser-based preparation, manipulation, and readout of the states of quantum particles has become a powerful research tool that has enabled the most precise measurements of time, fundamental constants, and electromagnetic fields. Laser…

Quantum Physics · Physics 2018-12-12 O. Schwartz , J. J. Axelrod , S. L. Campbell , C. Turnbaugh , R. M. Glaeser , H. Müller

Photoelectron holography constitutes a powerful tool for the ultrafast imaging of matter, as it combines high electron currents with subfemtosecond resolution, and gives information about transition amplitudes and phase shifts. Similarly to…

Atomic Physics · Physics 2020-02-19 C. Figueira de Morisson Faria , A. S. Maxwell

Electron ptychography provides highly sensitive, dose efficient phase images which can be corrected for aberrations after the data has been acquired. This is crucial when very precise quantification is required, such as with sensitivity to…

Materials Science · Physics 2023-07-27 Christoph Hofer , Timothy J. Pennycook

Spiral phase contrast is an important and convenient imaging processing technology in edge detection, and a broader field-of-view (FOV) of imaging is a long-pursuing aim to see more regions of the illumination objects. Compared with…

In this paper, we propose a new model to segment cells in phase contrast microscopy images. Cell images collected from the similar scenario share a similar background. Inspired by this, we separate cells from the background in images by…

Computer Vision and Pattern Recognition · Computer Science 2019-04-02 Lin Zhang

The transmission electron microscope facilitates the highest-resolution imaging of any instrument ever created, and its limiting factor is no longer spatial resolution but dose efficiency. Low electron doses avoid sample damage but produce…

Machine Learning · Computer Science 2026-05-08 Georgia Channing , Debora Keller , Marta D. Rossell , Philip Torr , Stig Helveg , Henrik Eliasson

First results on the experimental realisation of a 2x2 programmable phase plate for electrons are presented. The design consists of an array of electrostatic einzel lenses that influence the phase of electron waves passing through 4…

Existing Electric Field Conjugation (EFC) methods are not suited for treating small polarization effects, referred to here as cross polarization. EFC utilizes a deformable mirror (DM) to nullify the electric fields from the host star within…

Instrumentation and Methods for Astrophysics · Physics 2024-10-11 Richard A. Frazin

Cryo-electron microscopy can now routinely deliver atomic resolution structures for a variety of biological systems. The relevance and value of these structures is directly related to their ability to help rationalize experimental…

This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…

Quantitative Methods · Quantitative Biology 2023-11-02 Aniruddha Acharya

The numerical analysis of the diffraction features rendered by transmission electron microscopy (TEM) typically relies either on classical approximations (Monte Carlo simulations) or quantum paraxial tomography (the multislice method and…

Materials Science · Physics 2017-03-20 Samantha Rudinsky , Angel S. Sanz , Raynald Gauvin

The complex range of interactions between electrons and electromagnetic fields gave rise to countless scientific and technological advances. A prime example is photon-induced nearfield electron microscopy (PINEM), enabling the detection of…

A phase-sensitive microscopy technique is proposed and demonstrated that employs the momentum correlations inherent in spontaneous parametric down-conversion. One photon from a correlated pair is focused onto a microscopic target while the…

Quantum Physics · Physics 2023-01-23 Hazel Hodgson , Yingwen Zhang , Duncan England , Benjamin Sussman

Atomic-resolution cryogenic scanning transmission electron microscopy (cryo-STEM) has provided a path to probing the microscopic nature of select low-temperature phases in quantum materials. Expanding cryo-STEM techniques to broadly tunable…

Applied Physics · Physics 2020-11-05 Berit H. Goodge , Elisabeth Bianco , Henny W. Zandbergen Lena F. Kourkoutis

Correlative light-electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Most CLEM techniques use…

We present a novel approach for temporal contrast enhancement of energetic laserpulses by filtered SPM broadened spectra. A measured temporal contrast enhancement by at least 7 orders of magnitude in a simple setup has been achieved. This…

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