Related papers: Live-cell imaging powered by computation
Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…
Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…
Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…
Fluorescence microscopy, widely used in the study of living cells, tissues, and organisms, often faces the challenge of photodamage. This is primarily caused by the interaction between light and biochemical components during the imaging…
We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…
While high-resolution microscopic techniques are crucial for studying cellular structures in cell biology, obtaining such images from thick 3D engineered tissues remains challenging. In this review, we explore advancements in fluorescence…
We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…
High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…
A number of questions in systems biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently,…
Using bioimaging technology, biologists have attempted to identify and document analytical interpretations that underlie biological phenomena in biological cells. Theoretical biology aims at distilling those interpretations into knowledge…
Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…
Fluorescence microscopy has become a widely used tool for studying various biological structures of in vivo tissue or cells. However, quantitative analysis of these biological structures remains a challenge due to their complexity which is…
The study of biological systems witnessed a pervasive cross-fertilization between experimental investigation and computational methods. This gave rise to the development of new methodologies, able to tackle the complexity of biological…
4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail…
Automatic detection and tracking of cells in microscopy images are major applications of computer vision technologies in both biomedical research and clinical practice. Though machine learning methods are increasingly common in these…
An automatic approach to counting any kind of cells could alleviate work of the experts and boost the research in fields such as regenerative medicine. In this paper, a method for microscopy cell counting using multiple frames (hence…
Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…
Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…
We introduce a full field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering.…