Related papers: Fluorescence Lifetime Hong-Ou-Mandel Sensing
The lifetime of the $E^3\Pi_g(v=3)$ state of molecular iodine was measured in the gas phase at room temperature. The $E^3\Pi_g$ state was selectively populated by two sequential nanosecond pulse laser excitation. Resolved molecular…
Chlorophyll fluorescence (CF) is a key indicator to study plant physiology or photosynthesis efficiency. Conventionally, CF is characterized by fluorometers, which only allows ensemble measurement through wide-field detection. For imaging…
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…
Fluorescence lifetime imaging (FLI) has been receiving increased attention in recent years as a powerful diagnostic technique in biological and medical research. However, existing FLI systems often suffer from a tradeoff between processing…
In numerous industrial processes involving fluids, viscosity is a determinant factor for reaction rates, flows, drying, mixing, etc. Its importance is even more determinant for phenomena observed are at the micro- and nano- scales as in…
An approximation to coherent sampling, also known as boot-strapped waveform averaging, is presented. The method uses digital cavities to determine the condition for coherent sampling. It can be used to increase the effective sampling rate…
First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence…
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an…
Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…
Ultra-fast transmission electron microscopy (UTEM) combines sub-picosecond time-resolution with the versatility of TEM spectroscopies. It allows one to study the dynamics of materials properties combining complementary techniques. However,…
This paper concerns an inverse problem for fluorescence diffuse optical tomography (FDOT) reconstructing locations of multiple point targets from the measured temporal response functions. The targets are multiple fluorescent point objects…
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…
Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…
The large capacity and robustness of information encoding in the temporal mode of photons is important in quantum information processing, in which characterizing temporal quantum states with high usability and time resolution is essential.…
Microrheology, the study of fluids on micron length-scales, promises to reveal insights into cellular biology, including mechanical biomarkers of disease and the interplay between biomechanics and cellular function. Here a…
We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…
The concept and subsequent experimental verification of the proportionality between pulse amplitude and detector transit time for microchannel plate detectors is presented. This discovery has led to considerable improvement in the overall…
Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…
Real-time monitoring of dynamic biological processes in the body is critical to understanding disease progression and treatment response. This data, for instance, can help address the lower than 50% response rates to cancer immunotherapy.…
A system for time-correlated single photon counting applications based on CMOS single photon avalanche diodes is presented. An instrument response function of 79 ps allows a fluorescence decay of high-affinity Ca2+ indicator Oregon Green…