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Optical sections of the cornea are obtained by illumination with a collimated beam expanded in a fan shape by a small rotary cylindrical lens. The light diffused from the cornea is observed by two cameras and processed in order to yield the…

Medical Physics · Physics 2007-05-23 Jose B. Almeida , Sandra Franco

We propose an approach to far-field optical imaging beyond the diffraction limit. The proposed system allows image magnification, is robust with respect to material losses and can be fabricated by adapting existing metamaterial technologies…

Optics · Physics 2009-11-13 Zubin Jacob , Leonid V. Alekseyev , Evgenii Narimanov

We derive a formula for the light field of a monochromatic plane wave that is truncated and reflected by a spherical mirror. Our formula is valid even for deep mirrors, where the aperture radius approaches the radius of curvature. We apply…

Optics · Physics 2009-11-13 J. Goldwin , E. A. Hinds

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

Various methods have been introduced to measure the orbital angular momentum (OAM) of light, from fork holograms to Dove prism interferometers, from tilted lenses to triangular apertures - each with their own benefits and limitations. Here…

This paper demonstrates a method of interpreting the mechanism of aberration of optical systems based on non-Fourier transform optical transfer function (OTF). According to the parameters of object plane cosine fringe, we obtain the…

Optics · Physics 2018-12-27 Yu Bai , Jiaqi Chen , Fangjie Li , Zhenming Zhao

In some applications, the object space of light field imaging system is restrictive, such as industrial and medical endoscopes. If the traditional light field imaging system is used in the restrictive object space (ROS) directly but without…

Optics · Physics 2023-12-05 Ping Zhou , Nuo Chen , Yuda Xu , Chengcai Xu

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor…

Image and Video Processing · Electrical Eng. & Systems 2020-10-13 Kyrollos Yanny , Nick Antipa , William Liberti , Sam Dehaeck , Kristina Monakhova , Fanglin Linda Liu , Konlin Shen , Ren Ng , Laura Waller

Intensity, wavevector, phase, and polarization are the most important parameters of any light beam. Understanding the wavevector distribution has emerged as a very important problem in recent days, especially at nanoscale. It provides…

Optics · Physics 2020-01-22 Adarsh B. Vasista , Deepak K. Sharma , G V Pavan Kumar

The near fields of small-size extended hemielliptic lenses made of rexolite and isotropic quartz and illuminated by E- and H-polarized plane waves are studied. Variations in the focal domain size, shape, and location are presented versus…

Optics · Physics 2015-05-18 A. V. Boriskin , R. Sauleau , A. I. Nosich

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

We describe a new data collection scheme for optical diffusion tomography in which plane wave illumination is combined with multiple projections in the slab imaging geometry. Multiple projection measurements are performed by rotating the…

Medical Physics · Physics 2007-05-23 Vadim A. Markel , John C. Schotland

Compressive spectral imaging enables to reconstruct the entire three-dimensional (3D) spectral cube from a few multiplexed images. Here, we develop a novel compressive spectral imaging technique using diffractive lenses. Our technique uses…

Image and Video Processing · Electrical Eng. & Systems 2019-09-24 Oğuzhan Fatih Kar , Figen S. Oktem

We report on a new x-ray imaging method, which combines the high spatial resolution of coherent diffraction imaging with the ability of dark field microscopy to map grains within thick polycrystalline specimens. An x-ray objective serves to…

Instrumentation and Detectors · Physics 2020-07-15 Anders Filsøe Pedersen , Virginie Chamard , Carsten Detlefs , Tao Zhou , Dina Carbone , Henning Friis Poulsen

Spectroscopic photoemission microscopy is a well-established method to investigate the electronic structure of surfaces. In modern photoemission microscopes the electron optics allows imaging of the image plane, momentum plane, or…

Robust and accurate camera calibration is essential for 3D reconstruction in light microscopy under circular motion. Conventional methods require either accurate key point matching or precise segmentation of the axial-view images. Both…

Computer Vision and Pattern Recognition · Computer Science 2019-10-31 Yuanhao Guo , Fons J. Verbeek , Ge Yang

Despite recent advances, estimating optical flow remains a challenging problem in the presence of illumination change, large occlusions or fast movement. In this paper, we propose a novel optical flow estimation framework which can provide…

Computer Vision and Pattern Recognition · Computer Science 2018-04-12 Inchul Choi , Arunava Banerjee

Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has…

Optics · Physics 2015-03-13 Tim N. Ford , Kengyeh K. Chu , Jerome Mertz