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A microscope based on the Linnik interferometer was designed, built, and tested. Two methods were used for interference pattern measurement: phase-shifting and polarized single-shot methods. The former uses a low coherence light emitting…

Optics · Physics 2024-06-25 Sergei V. Anishchik , Marcos Dantus

Advances in fluorescence microscopy have reached a plateau in improving depth-to-resolution ratios for imaging scattering tissues, highlighting an urgent need for innovative techniques. We introduce Wavelens, a groundbreaking opaque lens…

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

Instrumentation and Detectors · Physics 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

We implemented the inverse design method to build a thin near-field lens that could produce a desired subwavelength focus by manipulating the near fields of a magnetic dipole source. The flat near-field lens represented by an artificial…

Optics · Physics 2015-06-16 Lu Lan , Wei Jiang , Yungui Ma

We describe several techniques developed by the High Resolution Fly's Eye experiment for measuring aerosol vertical optical depth, aerosol horizontal attenuation length, and aerosol phase function. The techniques are based on measurements…

Astrophysics · Physics 2012-08-27 The HiRes Collaboration

We present methods for optimizing pupil and focal plane optical elements that improve the performance of vortex coronagraphs on telescopes with obstructed or segmented apertures. Phase-only and complex masks are designed for the entrance…

Instrumentation and Methods for Astrophysics · Physics 2015-09-22 Garreth J. Ruane , Olivier Absil , Elsa Huby , Dimitri Mawet , Christian Delacroix , Brunella Carlomagno , Pierre Piron , Grover A. Swartzlander

Two transformation-optics inspired flat lenses are used to build up an optical system capable to transpose an area surrounding the object focal point in a magnified area surrounding the image focal point. The object and image focal points…

Optics · Physics 2018-08-10 Mircea Giloan , Robert Gutt

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Ptychography is a promising phase retrieval technique for visible light, X-ray and electron beams. Conventional ptychography reconstructs the amplitude and phase of an object light from a set of the diffraction intensity patterns obtained…

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

We present a novel learning-based method to build a differentiable computational model of a real fluorescence microscope. Our model can be used to calibrate a real optical setup directly from data samples and to engineer point spread…

Image and Video Processing · Electrical Eng. & Systems 2023-06-12 Josue Page , Paolo Favaro

An holographic microscopy reconstruction method compatible with high numerical aperture microscope objective (MO) up to NA=1.4 is proposed. After off axis and reference field curvature corrections, and after selection of the +1 grating…

Optics · Physics 2015-11-16 Nicolas Verrier , Dario Donnarumma , Gilles Tessier , Michel Gross

Capturing more information, e.g. geometry and material, using optical cameras can greatly help the perception and understanding of complex scenes. This paper proposes a novel method to capture the spectral and light field information…

Image and Video Processing · Electrical Eng. & Systems 2018-02-27 Qian Huang , Yunqian Li , Linsen Chen , Xiaoming Zhong , Jinli Suo , Zhan Ma , Tao Yue , Xun Cao

Future large space telescopes will be equipped with adaptive optics (AO) to overcome wavefront aberrations and achieve high contrast for imaging faint astronomical objects, such as earth-like exoplanets and debris disks. In contrast to AO…

Instrumentation and Methods for Astrophysics · Physics 2020-01-07 He Sun , N. Jeremy Kasdin , Robert Vanderbei

High-resolution optical imaging methods, such as confocal microscopy and full-field optical coherence tomography, capture flat optical sections of the sample. If the sample is curved, the optical field sections through several sample layers…

Medical Physics · Physics 2020-07-31 Viacheslav Mazlin , Kristina Irsch , Michel Paques , Mathias Fink , Albert Claude Boccara

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Visualizing the spatiotemporal evolution of the electric field of light is fundamental to optics, from designing photonic devices to developing next-generation microscopes. However, we lack the experimental tools to directly access the…

Optics · Physics 2026-03-04 Arjun Ashoka , Juhwan Lim , Akshay Rao , Dmitry A. Zimin

There are several methods to focus light behind a scattering medium, but very few use fluorescence light as feedback or can be used without access to the distal side of the scatterer. Among all the wave-front shaping techniques, retrieving…

Optics · Physics 2020-03-18 Evolene Premillieu , Rafael Piestun

The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…