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We present a single-image numerical phase retrieval method for Zernike phase-contrast microscopy (ZPM) that addresses halo and shade-off artifacts, as well as the weak phase condition, without requiring hardware modifications. By employing…

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful…

We report a compact, cost-effective and field-portable lensless imaging platform for quantitative microscopy. In this platform, the object is placed on top of an image sensor chip without using any lens. We use a low-cost galvo scanner to…

Image and Video Processing · Electrical Eng. & Systems 2019-04-26 He Zhang , Zichao Bian , Shaowei Jiang , Jian Liu , Pengming Song , Guoan Zheng

In the field of quantitative imaging, the image information at a pixel or voxel in an underlying domain entails crucial information about the imaged matter. This is particularly important in medical imaging applications, such as…

Optimization and Control · Mathematics 2024-04-12 Guozhi Dong , Moritz Flaschel , Michael Hintermüller , Kostas Papafitsoros , Clemens Sirotenko , Karsten Tabelow

A major hurdle in Quantum Image Processing (QIMP) is efficiently transferring classical, high-dimensional image data into quantum states. Current methods face trade-offs: amplitude encoding (FRQI) is computationally expensive in gate…

The spiral phase contrast microscope can clearly distinguish the morphological information of the low contrast objects (i.e., biological samples) because of the isotropic edge-enhancement effect, while the bright field microscope can image…

Optics · Physics 2024-02-20 Chuang Sun , Hailong Pi , Kian Shen Kiang , Jize Yan , Jun-Yu Ou

Complex-field imaging is indispensable for numerous applications at wavelengths from X-ray to THz, with amplitude describing transmittance (or reflectivity) and phase revealing intrinsic structure of the target object. Coherent diffraction…

Image and Video Processing · Electrical Eng. & Systems 2021-04-07 Meng Li , Liheng Bian , Guoan Zheng , Andrew Maiden , Yang Liu , Yiming Li , Qionghai Dai , Jun Zhang

While characterization of coherent wavefields is essential to laser, x-ray and electron imaging, sensors measure the squared magnitude of the field, rather than the field itself. Holography or phase retrieval must be used to characterize…

Image and Video Processing · Electrical Eng. & Systems 2020-12-10 David J. Brady , Timothy J. Schulz , Chengyu Wang

Optical metasurfaces have emerged as a new generation of building blocks for multi-functional optics. Design and realization of metasurface elements place ever-increasing demands on accurate assessment of phase alterations introduced by…

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Quantum phase is not a direct observable and is usually determined by interferometric methods. We present a method to map complete electron wave functions, including internal quantum phase information, from measured single-state probability…

Mesoscale and Nanoscale Physics · Physics 2008-03-19 Christopher R. Moon , Laila S. Mattos , Brian K. Foster , Gabriel Zeltzer , Wonhee Ko , Hari C. Manoharan

Quantitative phase imaging (QPI) is a valuable label-free modality that has gained significant interest due to its wide potentials, from basic biology to clinical applications. Most existing QPI systems measure microscopic objects via…

Optics · Physics 2021-12-22 Chenfei Hu , Mikhail E. Kandel , Young Jae Lee , Gabriel Popescu

Quantitative Magnetic Resonance Imaging (MRI) is based on a two-steps approach: estimation of the magnetic moments distribution inside the body, followed by a voxel-by-voxel quantification of the human tissue properties. This splitting…

There is a growing interest in reconstructing the density matrix of photoelectron wavepackets, in particular in complex systems where decoherence can be introduced either by a partial measurement of the system or through coupling with a…

We present a technically simple implementation of quantitative phase imaging in confocal microscopy based on synthetic optical holography with sinusoidal-phase reference waves. Using a Mirau interference objective and low-amplitude vertical…

Quantitative phase microscopy (QPM) is a label-free technique that enables to monitor morphological changes at subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence…

Scanning tunneling microscopy (STM) is a notoriously slow technique; Data-recording is serial which renders complex measurement tasks, such as quasiparticle interference (QPI) mapping, impractical. However, QPI would provide insight into…

Other Condensed Matter · Physics 2020-05-06 Jens Oppliger , Fabian Donat Natterer

Metalenses, owing to their ultra-thin planar structures, present a promising solution for reducing endoscopic invasiveness. However, achieving high-quality imaging with minimal invasiveness (short focal length of metalens) remains a…

We study the role of quantum entanglement (particle entanglement and mode entanglement) in optical phase estimation by employing the first and second quantization formalisms of quantum mechanics. The quantum Fisher information (QFI) is…

Quantum Physics · Physics 2016-09-23 Jaspreet Sahota , Nicolás Quesada , Daniel F. V. James

Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the…

Quantitative Methods · Quantitative Biology 2018-04-27 Paul Müller , Mirjam Schürmann , Salvatore Girardo , Gheorghe Cojoc , Jochen Guck