Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging
Quantitative Methods
2018-04-27 v2 Biological Physics
Abstract
Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the object's radius with geometrical approaches, neglecting light-scattering. Here, we present a novel QPI fitting algorithm that reliably uncouples the RI using Mie theory and a semi-analytical, corrected Rytov approach. We assess the range of validity of this algorithm in silico and experimentally investigate various objects (oil and protein droplets, microgel beads, cells) and noise conditions. In addition, we provide important practical cues for future studies in cell biology.
Cite
@article{arxiv.1803.06410,
title = {Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging},
author = {Paul Müller and Mirjam Schürmann and Salvatore Girardo and Gheorghe Cojoc and Jochen Guck},
journal= {arXiv preprint arXiv:1803.06410},
year = {2018}
}
Comments
14 pages, 10 figures, 1 table