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Histopathological image segmentation is a laborious and time-intensive task, often requiring analysis from experienced pathologists for accurate examinations. To reduce this burden, supervised machine-learning approaches have been adopted…

Computer Vision and Pattern Recognition · Computer Science 2024-09-12 Vishnuvardhan Purma , Suhas Srinath , Seshan Srirangarajan , Aanchal Kakkar , Prathosh A. P

Spatial transcriptomics (ST) enables the visualization of gene expression within the context of tissue morphology. This emerging discipline has the potential to serve as a foundation for developing tools to design precision medicines.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-12 Shivam Kumar , Samrat Chatterjee

Here, we present a diffusion transformer (DiT)-based pixel super-resolution virtual staining approach to transform low-resolution FTIR microspectroscopic images of the unstained tissues into corresponding high-resolution H&E-stained images.…

Optics · Physics 2026-03-10 Yudong Tian , Xiangyu Zhao , Yuqing Liu , Bofei Yang , Chongzhao Wu

We present a rapid, large-field bimodal imaging platform that integrates conventional brightfield microscopy with a lensless IR imaging scanner, enabling whole-slide IR image stack acquisition in minutes. Using a dedicated deep learning…

Virtual staining has emerged as a powerful alternative to traditional histopathological staining techniques, enabling rapid, reagent-free image transformations. However, existing evaluation methods predominantly rely on full-reference image…

Image and Video Processing · Electrical Eng. & Systems 2025-07-18 Qiankai Wang , James E. D. Tweel , Parsin Haji Reza , Anita Layton

Compared to hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) not only maintains the structural features of tissue samples, but also provides high-resolution protein localization, which is essential for aiding in pathology…

Computer Vision and Pattern Recognition · Computer Science 2025-11-04 Yuhang Kang , Ziyu Su , Tianyang Wang , Zaibo Li , Wei Chen , Muhammad Khalid Khan Niazi

Medical Image Synthesis (MIS) plays an important role in the intelligent medical field, which greatly saves the economic and time costs of medical diagnosis. However, due to the complexity of medical images and similar characteristics of…

Image and Video Processing · Electrical Eng. & Systems 2025-03-07 Zhihan Ju , Wanting Zhou , Longteng Kong , Yu Chen , Yi Li , Zhenan Sun , Caifeng Shan

There is a significant need for the generation of virtual histological information from coronary optical coherence tomography (OCT) images to better guide the treatment of coronary artery disease. However, existing methods either require a…

Image and Video Processing · Electrical Eng. & Systems 2023-07-25 Xueshen Li , Hongshan Liu , Xiaoyu Song , Brigitta C. Brott , Silvio H. Litovsky , Yu Gan

The rapid development of digital pathology and modern deep learning has facilitated the emergence of pathology foundation models that are expected to solve general pathology problems under various disease conditions in one unified model,…

Computer Vision and Pattern Recognition · Computer Science 2025-07-10 Yutong Sun , Sichen Zhu , Peng Qiu

Staining is essential in cell imaging and medical diagnostics but poses significant challenges, including high cost, time consumption, labor intensity, and irreversible tissue alterations. Recent advances in deep learning have enabled…

Computer Vision and Pattern Recognition · Computer Science 2025-04-15 Ziwang Xu , Lanqing Guo , Satoshi Tsutsui , Shuyan Zhang , Alex C. Kot , Bihan Wen

Although generative adversarial network (GAN) based style transfer is state of the art in histopathology color-stain normalization, they do not explicitly integrate structural information of tissues. We propose a self-supervised approach to…

Image and Video Processing · Electrical Eng. & Systems 2021-06-04 Dwarikanath Mahapatra , Behzad Bozorgtabar , Jean-Philippe Thiran , Ling Shao

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori

Immunohistochemical (IHC) staining provides crucial molecular characterization of tissue samples and plays an indispensable role in the clinical examination and diagnosis of cancers. However, compared with the commonly used Hematoxylin and…

Computer Vision and Pattern Recognition · Computer Science 2026-01-07 Mingzhou Jiang , Jiaying Zhou , Nan Zeng , Mickael Li , Qijie Tang , Chao He , Huazhu Fu , Honghui He

In digital pathology, many image analysis tasks are challenged by the need for large and time-consuming manual data annotations to cope with various sources of variability in the image domain. Unsupervised domain adaptation based on…

Image and Video Processing · Electrical Eng. & Systems 2022-05-18 Nassim Bouteldja , Barbara Mara Klinkhammer , Tarek Schlaich , Peter Boor , Dorit Merhof

Unsupervised and unpaired domain translation using generative adversarial neural networks, and more precisely CycleGAN, is state of the art for the stain translation of histopathology images. It often, however, suffers from the presence of…

Computer Vision and Pattern Recognition · Computer Science 2022-07-04 Nicolas Brieu , Felix J. Segerer , Ansh Kapil , Philipp Wortmann , Guenter Schmidt

"Is it possible to predict expression levels of different genes at a given spatial location in the routine histology image of a tumor section by modeling its stain absorption characteristics?" In this work, we propose a "stain-aware"…

Image and Video Processing · Electrical Eng. & Systems 2021-08-27 Muhammad Dawood , Kim Branson , Nasir M. Rajpoot , Fayyaz ul Amir Afsar Minhas

Segmentation from renal pathological images is a key step in automatic analyzing the renal histological characteristics. However, the performance of models varies significantly in different types of stained datasets due to the appearance…

Image and Video Processing · Electrical Eng. & Systems 2020-02-21 Ke Mei , Chuang Zhu , Lei Jiang , Jun Liu , Yuanyuan Qiao

The differentiation between pathological subtypes of non-small cell lung cancer (NSCLC) is an essential step in guiding treatment options and prognosis. However, current clinical practice relies on multi-step staining and labelling…

Quantitative Methods · Quantitative Biology 2026-03-10 Zhenya Zang , David A Dorward , Katherine E Quiohilag , Andrew DJ Wood , James R Hopgood , Ahsan R Akram , Qiang Wang

Hematoxylin and eosin (H&E) staining is the clinical standard for assessing tissue morphology, but it lacks molecular-level diagnostic information. In contrast, immunohistochemistry (IHC) provides crucial insights into biomarker expression,…

Image and Video Processing · Electrical Eng. & Systems 2025-08-05 Jingsong Liu , Xiaofeng Deng , Han Li , Azar Kazemi , Christian Grashei , Gesa Wilkens , Xin You , Tanja Groll , Nassir Navab , Carolin Mogler , Peter J. Schüffler

Precise segmentation and classification of cell instances are vital for analyzing the tissue microenvironment in histology images, supporting medical diagnosis, prognosis, treatment planning, and studies of brain cytoarchitecture. However,…

Computer Vision and Pattern Recognition · Computer Science 2025-02-14 Valentina Vadori , Jean-Marie Graïc , Antonella Peruffo , Livio Finos , Ujwala Kiran Chaudhari , Enrico Grisan
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