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Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous…

Optics · Physics 2024-09-04 Carlo Bevilacqua , Robert Prevedel

Brillouin microscopy is an emerging label-free imaging technique to assess local viscoelastic properties. Quantum-enhanced stimulated Brillouin scattering is demonstrated for the first time using low power continuous-wave lasers at 795~nm.…

Optics · Physics 2024-09-16 Tian Li , Fu Li , Xinghua Liu , Vladislav V. Yakovlev , Girish S. Agarwal

The role and importance of mechanical properties of cells and tissues in cellular function, development as well as disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic…

Biological Physics · Physics 2019-01-09 Robert Prevedel , Alba Diz-Muñoz , Giancarlo Ruocco , Giuseppe Antonacci

Brillouin spectroscopy and imaging are emerging techniques in analytical science, biophotonics and biomedicine. They are based on Brillouin light scattering from acoustic waves or phonons in the GHz range, providing a nondestructive…

Biological Physics · Physics 2021-10-12 Francesca Palombo , Daniele Fioretto

Brillouin microscopy is rapidly emerging as a powerful technique for imaging the mechanical properties of biological specimens in a label-free, non-contact manner. We present a standardized file format and open-source tools to facilitate…

Brillouin imaging is increasingly recognized to be a powerful technique that enables non-invasive measurement of the mechanical properties of cells and tissues on a microscopic scale. This provides an unprecedented tool for investigating…

Biological Physics · Physics 2020-01-22 Christine Poon , Joshua Chou , Michael Cortie , Irina Kabakova

Brillouin spectroscopy is a century-old technique that has recently received renewed interest, as modern instrumentation has transformed it into a powerful contactless and label-free probe of micromechanical properties for biomedical…

Instrumentation and Detectors · Physics 2020-05-27 YuChen Xiang , Carin Basirun , Joshua Chou , Majid E. Warkiani , Peter Török , Yingying Wang , Shoufei Gao , Irina V. Kabakova

Brillouin Light Scattering is a powerful technique to measure the microwave excitations present in a magnetic system. In microfocused mode, the light is focused on the sample using a microscope objective. This accelerates substantially the…

Mesoscale and Nanoscale Physics · Physics 2025-06-30 Nessrine Benaziz , Thibaut Devolder , Jean-Paul Adam

The biomechanical properties of cells and tissues play an important role in our fundamental understanding of the structures and functions of biological systems at both the cellular and subcellular levels. Recently, Brillouin microscopy,…

Stimulated Brillouin interactions mediate nonlinear coupling between photons and acoustic phonons through an optomechanical three-wave interaction. Though these nonlinearities were previously very weak in silicon photonic systems, the…

Optics · Physics 2017-08-02 Eric A. Kittlaus , Nils T. Otterstrom , Peter T. Rakich

Many problems in mechanobiology urgently require characterisation of the micromechanical properties of the fibrous proteins of cells and tissues. Brillouin light scattering has been proposed as a new optical elastography technique to meet…

Characterizing the micromechanical properties of cells and extracellular matrices is critical in mechanobiology. To meet this need, Brillouin light scattering (BLS) has emerged as a noncontact, high-resolution elastography tool that probes…

Noncontact label-free biomechanical imaging is a crucial tool for unraveling the mechanical properties of biological systems, which play critical roles in the fields of engineering, physics, biology and medicine; yet, it represents a…

Biological Physics · Physics 2020-09-29 Itay Remer , Netta Shemsesh , Anat Ben-Zvi , Alberto Bilenca

Optical imaging with mechanical contrast is critical for material and biological discovery since it allows contactless light-radiation force-excitation within the sample, as opposed to traditional mechanical imaging. Whilst optical…

Brillouin imaging relies on the reliable extraction of subtle spectral information from hyperspectral datasets. To date, the mainstream practice has been using line fitting of spectral features to retrieve the average peak shift and…

Image and Video Processing · Electrical Eng. & Systems 2021-04-07 YuChen Xiang , Kai Ling C. Seow , Carl Paterson , Peter Török

Brillouin Microscopy is a novel label-free optical technique that enables the measurement of a material's mechanical properties at the sub-micron scale in a non-invasive and non-contact way; in the last few years, its applications in the…

Noncontact Brillouin spectroscopy is a purely optical and label-free method to retrieve fundamental material viscoelastic properties. Recently, the extension to a three-dimensional imaging modality has paved the way to novel exciting…

Optics · Physics 2022-02-24 Giuseppe Antonacci , Kareem Elsayad , Dario Polli

Brillouin microscopy is an emerging optical technique for probing mechanical properties with submicron resolution, offering fully non-contact, label-free operation. Despite its unique capabilities, broader adoption has been limited by slow…

Optics · Physics 2026-04-22 Mikolaj Pochylski

Brillouin microscopy, which maps elastic modulus from the frequency shift of scattered light, has evolved to a faster speed for the investigation of rapid biomechanical changes. Impulsive stimulated Brillouin scattering (ISBS) spectroscopy…

Optics · Physics 2023-12-07 Jiarui Li , Taoran Le , Hongyuan Zhang , Haoyun Wei , Yan Li
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