Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials
Abstract
Brillouin Light Scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the sub-micron scale. Over the last decade it has seen increased applications in the life sciences, driven by the observed significance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and its extension to an imaging modality. As with other spectroscopic techniques, BLS measurements not only detect signals characteristic of the investigated sample, but also of the experimental apparatus, and can be significantly affected by measurement conditions. The aim of this consensus statement is to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artifacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different--often self-built--spectrometers, a consensus statement is particularly timely to assure unified advancement.
Keywords
Cite
@article{arxiv.2411.11712,
title = {Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials},
author = {Pierre Bouvet and Carlo Bevilacqua and Yogeshwari Ambekar and Giuseppe Antonacci and Joshua Au and Silvia Caponi and Sophie Chagnon-Lessard and Juergen Czarske and Thomas Dehoux and Daniele Fioretto and Yujian Fu and Jochen Guck and Thorsten Hamann and Dag Heinemann and Torsten Jähnke and Hubert Jean-Ruel and Irina Kabakova and Kristie Koski and Nektarios Koukourakis and David Krause and Salvatore La Cavera and Timm Landes and Jinhao Li and Jeremie Margueritat and Maurizio Mattarelli and Michael Monaghan and Darryl R. Overby and Fernando Perez-Cota and Emanuele Pontecorvo and Robert Prevedel and Giancarlo Ruocco and John Sandercock and Giuliano Scarcelli and Filippo Scarponi and Claudia Testi and Peter Török and Lucie Vovard and Wolfgang Weninger and Vladislav Yakovlev and Seok-Hyun Yun and Jitao Zhang and Francesca Palombo and Alberto Bilenca and Kareem Elsayad},
journal= {arXiv preprint arXiv:2411.11712},
year = {2024}
}
Comments
Main Text & Supplementary Text: 56 pages, 3 Figures, 2 Supplementary Figures, 1 Supplementary Table