Related papers: A statistical resolution measure of fluorescence m…
Optical microscopy is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical…
As a general rule of thumb the resolution of a light microscope (i.e. the ability to discern objects) is predominantly described by the full width at half maximum (FWHM) of its point spread function (psf)---the diameter of the blurring…
Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…
Superresolution fluorescence microscopy techniques beat the diffraction limit, enabling ultra-high resolution imaging in biological physics and nanoscience. In all cases that have been studied experimentally, the resolution scales inversely…
We realize a scanning probe microscope using single trapped $^{87}$Rb atoms to measure optical fields with subwavelength spatial resolution. Our microscope operates by detecting fluorescence from a single atom driven by near-resonant light…
We derive the fundamental limit to the resolution of far-field optical imaging, and demonstrate that, while a bound to the resolution of a fundamental nature does exit, contrary to the conventional wisdom it is neither exactly equal to nor…
The optical diffraction limit, formulated by Abbe 140 years ago, imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in…
In recent years several methods to overcome diffraction limit in the far field microscopy have been demonstrated. Still the problem of superresolution is reliably solved only for fluorescent microscopy, giving a resolution of up to 20-30nm.…
Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…
Abbe's resolution limit, one of the best-known physical limitations, poses a great challenge for any wave systems in imaging, wave transport, and dynamics. Originally formulated in linear optics, this Abbe's limit can be broken using…
The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…
In recent years several far-field microscopy techniques have been developed which manage to overcome the diffraction limit of resolution. A unifying classification scheme for them is clearly desirable. We argue that existing schemes based…
For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial…
Interference of light fields plays an important role in various high-precision measurement schemes. It has been shown that super resolving phase measurements beyond the standard coherent state limit can be obtained either by using maximally…
We experimentally demonstrate quantum enhanced resolution in confocal fluorescence microscopy exploiting the non-classical photon statistics of single nitrogen-vacancy colour centres in diamond. By developing a general model of…
Precision measurement has been an important research area in sensing and metrology. In classical physics, the Fisher information determines the maximum extractable information from statistically unknown signals, based on a joint probability…
The resolution of far-field optical microscopes, which rely on propagating optical modes, is widely believed to be limited because of diffraction to a value on the order of a half-wavelength $\lambda /2$ of the light used. Although…
The ultimate sensitivity of optical measurements is a key element of many recent works. Classically, it is mainly limited by the shot noise limit. However, a measurement setup that incorporates quantum mechanical principles can surpass the…
A new far-field optical microscopy technique capable of reaching nanometer-scale resolution has been developed recently using the in-plane image magnification by surface plasmon polaritons. This microscopy is based on the optical properties…
Imaging of rapidly occurring events in biology requires high temporal resolution. In this review article, I have tried to investigate an approximate estimate to quantify temporal resolution limit of a fluorescence imaging system. It was…