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Single-molecule fluorescence techniques have revolutionized our ability to study proteins. However, the presence of a fluorescent label can alter the protein structure and/or modify its reaction with other species. To avoid the need for a…

Biological Physics · Physics 2022-04-07 Aleksandr Barulin , Prithu Roy , Jean-Benoît Claude , Jérôme Wenger

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Interferometric-based microscopies stand as powerful label-free approaches for monitoring and characterising chemical reactions and heterogeneous nanoparticle systems in real time with single particle sensitivity. Nevertheless, coherent…

We propose and numerically demonstrate a technique for subwavelength imaging based on a metal-dielectric multilayer hyperlens designed in such a way that only the large-wavevector waves are transmitted while all propagating waves from the…

Optics · Physics 2015-10-28 Taavi Repän , Andrei V. Lavrinenko , Sergei V. Zhukovsky

Infrared spectroscopy, especially for molecular vibrations in the fingerprint region between 600 and 1500 cm-1, is a powerful characterization method for bulk materials. However, molecular fingerprinting at the nanoscale level still remains…

Optics · Physics 2016-08-24 Hai Hu , Xiaoxia Yang , Feng Zhai , Debo Hu , Ruina Liu , Kaihui Liu , Zhipei Sun , Qing Dai

Recently, deep learning-based methods achieved promising performance in nuclei detection and classification applications. However, training deep learning-based methods requires a large amount of pixel-wise annotated data, which is…

Image and Video Processing · Electrical Eng. & Systems 2024-01-17 Nazanin Moradinasab , Rebecca A. Deaton , Laura S. Shankman , Gary K. Owens , Donald E. Brown

Lensfree on-chip microscopy is an emerging imaging technique that can be used to visualize and study biological specimens without the need for imaging lens systems. Important issues that can limit the performance of lensfree on-chip…

Optics · Physics 2015-05-11 Alexander Wong , Farnoud Kazemzadeh , Chao Jin , Xiao Yu Wang

Label-free spectromicroscopy methods offer the capability to examine complex cellular phenomena. Electron and X-ray-based spectromicroscopy methods, though powerful, have been hard to implement with hydrated objects due to the vacuum…

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

Optics · Physics 2025-01-22 Jaromír Běhal , Miroslav Ježek

To overcome the resolution limit of conventional optics, near field imaging techniques using a negative index flat lens (NIFL) have been previously developed that amplify the evanescent components of the incident field. Here, a technique is…

Optics · Physics 2016-12-23 Wyatt Adams , Durdu Ö. Güney

Interaction-free measurement (IFM) has been proposed as a means of high-resolution, low-damage imaging of radiation-sensitive samples, such as biomolecules and proteins. The basic setup for IFM is a Mach-Zehnder interferometer, and recent…

Instrumentation and Detectors · Physics 2019-06-12 Akshay Agarwal , Karl K. Berggren , Vivek Goyal

Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit…

Methods for nanoscale material characterization are in ever-increasing demand, especially those that can provide a broader range of information at once. Near-field techniques based on combinations of scanning probe microscopy (SPM) and…

Applied Physics · Physics 2020-11-11 Alvaro Rodriguez , Tim Verhagen , Martin Kalbac , Jana Vejpravova , Otakar Frank

The ability to engineer localized surface plasmon resonances at large scale usually relies on precise nanoscale patterning. Here, we demonstrate that mid-infrared plasmonic responses can instead emerge in unpatterned polysilicon films…

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…

Metallic nanostructures provide a toolkit for the generation of coherent light below the diffraction limit. Plasmonic based lasing relies on the population inversion of emitters (such as organic fluorophores) along with feedback provided by…

The ability to use coherent light for material science and applications is directly linked to our ability to measure short optical pulses. While free-space optical methods are well-established, achieving this on a chip would offer the…

Label-free detection techniques for single particles and molecules play an important role in basic science, disease diagnostics, and nanomaterial investigations. While traditional fluorescence-based methods offer powerful tools for single…

Optics · Physics 2024-08-20 Shuang Hao , Sartanee Suebka , Judith Su

The flexible endoscope is a minimally invasive tool in clinical settings, but most of them rely on exogenous staining for diagnosis to provide qualitative information. Here, we demonstrated a flexible endoscopic microscopy (FEM) with…

Non-invasive optical focusing inside scattering media is still a big challenge because inhomogeneous media scatter both incoming photons for focusing and outgoing photons for observation. Various approaches, utilizing non-linear…

Optics · Physics 2021-09-17 Dayan Li , Sujit Kumar Sahoo , Huy Quoc Lam , Dong Wang , Cuong Dang