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Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…

Adaptive optics (AO) has revolutionized imaging in {fields} from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations due to the absence of guidestar and the need…

Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…

Optics · Physics 2025-09-23 Biwei Zhang , Qi Hu , Jingyu Wang , Martin J. Booth

Astronomical telescopes suffer from a tradeoff between field of view (FoV) and image resolution: increasing the FoV leads to an optical field that is under-sampled by the science camera. This work presents a novel computational imaging…

Instrumentation and Methods for Astrophysics · Physics 2025-08-07 Robin Swanson , Esther Y. H. Lin , Masen Lamb , Suresh Sivanandam , Kiriakos N. Kutulakos

Multifocal plane microscopy (MUM) allows three dimensional objects to be imaged in a single camera frame. Our approach uses dual orthogonal diffraction phase gratings with a quadratic distortion of the lines to apply defocus to the first…

Optics · Physics 2020-03-13 Benjamin Gore , Noah Schwartz , Paul Dalgarno

Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…

Optics · Physics 2026-03-04 Qi Hu , Jingyu Wang , Huriye Atilgan , Armin Lak , Martin J. Booth

Modern adaptive optics (AO) systems for large telescopes require tomographic techniques to reconstruct the phase aberrations induced by the turbulent atmosphere along a line of sight to a target which is angularly separated from the guide…

Instrumentation and Methods for Astrophysics · Physics 2015-06-03 James Osborn , Francisco Javier De Cos Juez , Dani Guzman , Timothy Butterley , Richard Myers , Andres Guesalaga , Jesus Laine

Adaptive optics (AO) is a powerful tool employed across various research fields, from aerospace to microscopy. Traditionally, AO has focused on correcting optical phase aberrations, with recent advances extending to polarisation…

Adaptive optics (AO) are reconfigurable devices that compensate for wavefront distortions or aberrations in optical systems such as microscopes, telescopes and ophthalmoscopes. Aberrations have detrimental effects that can reduce imaging…

Optics · Physics 2025-06-10 Biwei Zhang , Martin J. Booth , Qi Hu

This work introduces the first closed-loop adaptive optics (AO) system capable of optically correcting aberrations in real-time without a guidestar or a wavefront sensor. Nearly 40 years ago, Cederquist et al. demonstrated that asymmetric…

Image and Video Processing · Electrical Eng. & Systems 2026-05-14 Weiyun Jiang , Haiyun Guo , Christopher A. Metzler , Ashok Veeraraghavan

The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM…

Optics · Physics 2015-06-23 Jerome Mertz , Hari Paudel , Thomas G. Bifano

Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of…

Optics · Physics 2019-02-06 Lyubov V. Amitonova

Since the year 2000, adaptive optics (AO) has seen the emergence of a variety of new concepts addressing particular science needs; multiconjugate adaptive optics (MCAO) is one of them. By correcting the atmospheric turbulence in 3D using…

Instrumentation and Methods for Astrophysics · Physics 2020-03-09 Francois Rigaut , Benoit Neichel

Label-free tomographic microscopy offers a compelling means to visualize three-dimensional (3D) refractive index (RI) distributions from two-dimensional (2D) intensity measurements. However, limited forward-model accuracy and the ill-posed…

Optics · Physics 2025-02-27 Delong Yang , Shaohui Zhang , Jiasong Sun , Chao Zuo , Qun Hao

Adaptive optics (AO) offers an opportunity to stabilize an image and maximize the spatial resolution achievable by ground based telescopes by removing the distortions due to the atmosphere. Typically, the deformable mirror in an AO system…

Instrumentation and Methods for Astrophysics · Physics 2021-07-30 J. Fowler , Rachel Bowens-Rubin , Philip M. Hinz

One important frontier for astronomical adaptive optics (AO) involves methods such as Multi-Object AO and Multi-Conjugate AO that have the potential to give a significantly larger field of view than conventional AO techniques. A second key…

Instrumentation and Methods for Astrophysics · Physics 2015-05-13 S. Mark Ammons , Luke Johnson , Edward A. Laag , Renate Kupke , Donald T. Gavel , Brian J. Bauman , Claire E. Max

Adaptive optics normally concerns the feedback correction of phase aberrations. Such correction has been of benefit in various optical systems, with applications ranging in scale from astronomical telescopes to super-resolution microscopes.…

Optics · Physics 2021-10-07 Chao He , Jacopo Antonello , Martin J. Booth

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Control of light through a microscope objective with a high numerical aperture is a common requirement in applications such as optogenetics, adaptive optics, or laser processing. Light propagation, including polarization effects, can be…

Optics · Physics 2023-03-22 Ivan Vishniakou , Johannes D. Seelig

Radial velocity instruments require high spectral resolution and extreme thermo-mecanical stability, even more difficult to achieve in near-infra red (NIR) where the spectrograph has to be cooled down. For a seeing-limited spectrograph, the…

Instrumentation and Methods for Astrophysics · Physics 2016-11-23 Uriel Conod , Nicolas Blind , François Wildi , Francesco Pepe
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