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Human physiology and pathology arise from the coordinated interactions of diverse single cells. However, analyzing single cells has been limited by the low sensitivity and throughput of analytical methods. DNA sequencing has recently made…

Quantitative Methods · Quantitative Biology 2020-06-23 Nikolai Slavov

Nanopore-based sensing platforms have transformed single-molecule detection and analysis. The foundation of nanopore translocation experiments lies in conductance measurements, yet existing models, which are largely phenomenological, are…

Biological Physics · Physics 2023-12-19 Arjav Shah , Shakul Pathak , Slaven Garaj , Martin Z. Bazant , Ankur Gupta , Patrick S. Doyle

Low-energy electrons offer a unique possibility for long exposure imaging of individual biomolecules without significant radiation damage. In addition, low-energy electrons exhibit high sensitivity to local potentials and thus can be…

Mesoscale and Nanoscale Physics · Physics 2019-08-27 Tatiana Latychevskaia , Conrad Escher , William Andregg , Michael Andregg , Hans-Werner Fink

A nanoparticle detection scheme with single particle resolution is presented. The sensor contains only a taper fiber thus offering the advantages of compactness and installation flexibility. Sensing method is based on monitoring the…

Optics · Physics 2013-05-31 Jiangang Zhu , Sahin Kaya Ozdemir , Lan Yang

In this study, we examine the mechanism of nanopore-based DNA sequencing using a voltage bias across a graphene nanoribbon. Using density functional theory and a non-equilibrium Green's function approach, we determine the transmission…

Mesoscale and Nanoscale Physics · Physics 2018-09-11 Hannah L. McFarland , Towfiq Ahmed , Jian-Xin Zhu , Alexander V. Balatsky , Jason T. Haraldsen

Single particle tracking systems monitor cellular processes with great accuracy in nano-biological systems. The emissions of the fluorescent molecules are detected with cameras or photodetectors. However, state-of-the-art imaging systems…

Quantitative Methods · Quantitative Biology 2018-05-16 Burhan Gulbahar , Gorkem Memisoglu

Superconducting nanowire single photon detectors (SNSPD or SSPD) are highly promising devices in the growing field of quantum information and communications technology. We have developed a practical SSPD system with our superconducting thin…

Quantum Physics · Physics 2016-11-17 Zhen Wang , Shigehito Miki , Mikio Fujiwara

Nanopores in 2D materials are of significant interest in advanced membrane technologies aimed at the sensing and separation of ions and molecules. These applications necessitate 2D nanopores that are precise in size and shape, and abundant…

Materials Science · Physics 2024-08-20 Dana O. Byrne , Frances I. Allen

We demonstrate a new, versatile class of nanoscale chemical sensors based on single-stranded DNA (ss-DNA) as the chemical sensors recognition site and single-walled carbon nanotube field effect transistors (swCN-FET) as the electronic…

Materials Science · Physics 2007-05-23 Cristian Staii , Michelle Chen , Alan Gelperin , Alan T. Johnson

A general theoretical framework is put forth to organize and understand various observed phenomena and mathematical relationships in the field of molecular biology. By modeling each cell in eukaryotic organisms as a processor having a…

Other Quantitative Biology · Quantitative Biology 2013-12-18 Barry D. Jacobson

Nanopore sequencing, a next-generation sequencing technology, holds the potential to revolutionize multiple facets of life sciences, forensics, and healthcare. While previous research has focused on its technical intricacies and biomedical…

Physics and Society · Physics 2024-10-29 Suadath V , Muhammad Sajeer P

Ionic transport in nanopores or nanochannels is key to many cellular processes and is now being explored as a method for DNA/polymer sequencing and detection. Although apparently simple in its scope, the study of ionic dynamics in confined…

Mesoscale and Nanoscale Physics · Physics 2013-06-13 Andrew Meyertholen , Massimiliano Di Ventra

We present a simplified model of the dynamics of translocation of RNA through a nanopore which only allows the passage of unbound nucleotides. In particular, we consider the disorder averaged translocation dynamics of random, two-component,…

Biomolecules · Quantitative Biology 2007-05-23 J. B. Lucks , Y. Kafri

Single-photon detectors based on superconducting nanowires (SSPDs or SNSPDs) have rapidly emerged as a highly promising photon-counting technology for infrared wavelengths. These devices offer high efficiency, low dark counts and excellent…

Quantum Physics · Physics 2012-04-26 Chandra M. Natarajan , Michael G. Tanner , Robert H. Hadfield

Satellite-ground quantum communication requires single-photon detectors of 850-nm wavelength with both high detection efficiency and large sensitive area. We developed superconducting nanowire single-photon detectors (SNSPDs) on…

Both the translational diffusion coefficient $D$ and the electrophoretic mobility $\mu$ of a short rod-like molecule (such as dsDNA) that is being pulled towards a nanopore by an electric field should depend on its orientation. Since a…

Biological Physics · Physics 2020-06-25 Le Qiao , Gary W. Slater

Nanopore resistive pulse sensors are emerging technologies for single-molecule protein sequencing. But they can hardly detect small post-translational modifications (PTMs) such as hydroxylation in single-molecule level. While a combination…

Quantitative Methods · Quantitative Biology 2024-01-09 Mulusew W. Yaltaye , Yingqi Zhao , Eva Bozo , Pei-Lin Xin , Vahid Farrah , Francesco De Angelis , Jian-An Huang

Engineered electromagnetic fields in plasmonic nanopores enable enhanced optical detection and their use in single molecule sequencing. Here, a plasmonic nanopore prepared in a thick nanoporous film is used to investigate the interaction…

The translocation of structured RNA or DNA molecules through narrow pores necessitates the opening of all base pairs. Here, we study the interplay between the dynamics of translocation and base-pairing theoretically, using kinetic Monte…

Biomolecules · Quantitative Biology 2009-11-11 Ralf Bundschuh , Ulrich Gerland

Single molecule force spectroscopy of DNA strands adsorbed at surfaces is a powerful technique used in air or liquid environments to quantify their mechanical properties. Although the force responses are limited to unfolding events so far,…