lambda DNA through a plasmonic nanopore What can be detected by means of Surface Enhanced Raman Scattering?
Abstract
Engineered electromagnetic fields in plasmonic nanopores enable enhanced optical detection and their use in single molecule sequencing. Here, a plasmonic nanopore prepared in a thick nanoporous film is used to investigate the interaction between the metal and a long-chain double strand DNA molecule. We discuss how the matrix of nanoporous metal can interact with the molecule thanks to: i) transient aspecific interactions between the porous surface and DNA and ii) optical forces exerted by the localized field in a metallic nanostructure. A duration of interaction up to tens of milliseconds enables to collect high signal-to-noise Raman vibrations allowing an easy label-free reading of information from the DNA molecule. Moreover, in order to further increase the event of detection rate, we tested a polymeric porous hydrogel placed beneath the solid-state membrane. This approach enables a slowdown of the molecule diffusion, thus increasing the number of detected interactions by a factor of about 20.
Keywords
Cite
@article{arxiv.2004.00950,
title = {lambda DNA through a plasmonic nanopore What can be detected by means of Surface Enhanced Raman Scattering?},
author = {Aliaksandr Hubarevich and Jian-An Huang and Giorgia Giovannini and Andrea Schirato and Yingqi Zhao and Nicolò Maccaferri and Francesco De Angelis and Alessandro Alabastri and Denis Garoli},
journal= {arXiv preprint arXiv:2004.00950},
year = {2020}
}