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Advancements in cytometry technologies have led to a remarkable increase in the number of markers that can be analyzed simultaneously, presenting significant challenges in data analysis. Traditional approaches, such as dimensional reduction…

Quantitative Methods · Quantitative Biology 2025-07-01 Masahiro Ono

Analysis of flow cytometry data is an essential tool for clinical diagnosis of hematological and immunological conditions. Current clinical workflows rely on a manual process called gating to classify cells into their canonical types. This…

Machine Learning · Statistics 2017-11-29 Disi Ji , Eric Nalisnick , Padhraic Smyth

Single-cell RNA-seq (scRNA-seq) enables atlas-scale profiling of complex tissues, revealing rare lineages and transient states. Yet, assigning biologically valid cell identities remains a bottleneck because markers are tissue- and…

Flow cytometry mainly used for detecting the characteristics of a number of biochemical substances based on the expression of specific markers in cells. It is particularly useful for detecting membrane surface receptors, antigens, ions, or…

Machine Learning · Computer Science 2023-03-17 Yanhua Xu

Generative modeling of single-cell RNA-seq data is crucial for tasks like trajectory inference, batch effect removal, and simulation of realistic cellular data. However, recent deep generative models simulating synthetic single cells from…

Quantitative Methods · Quantitative Biology 2025-03-04 Alessandro Palma , Till Richter , Hanyi Zhang , Manuel Lubetzki , Alexander Tong , Andrea Dittadi , Fabian Theis

Automation in medical imaging is quite challenging due to the unavailability of annotated datasets and the scarcity of domain experts. In recent years, deep learning techniques have solved some complex medical imaging tasks like disease…

Image and Video Processing · Electrical Eng. & Systems 2024-03-19 Soumyajyoti Dey , Sukanta Chakraborty , Utso Guha Roy , Nibaran Das

Accurate and scalable cell type annotation remains a challenge in single-cell transcriptomics, especially when datasets exhibit strong batch effects or contain previously unseen cell populations. Here we introduce SpikGPT, a hybrid deep…

Quantitative Methods · Quantitative Biology 2025-12-04 Min Huang , Rishikesan Kamaleswaran

Modern optical microscopes are fully motorised; however, transforming them into truly smart systems requires real-time adjustment of acquisition settings in response to detected objects and dynamic biological events. At the core are…

Single-cell RNA sequencing has transformed our ability to identify diverse cell types and their transcriptomic signatures. However, annotating these signatures-especially those involving poorly characterized genes-remains a major challenge.…

Ocean microbes are critical to both ocean ecosystems and the global climate. Flow cytometry, which measures cell optical properties in fluid samples, is routinely used in oceanographic research. Despite decades of accumulated data,…

Methodology · Statistics 2025-04-17 Sangwon Hyun , Tim Coleman , Francois Ribalet , Jacob Bien

In recent years, single cell RNA sequencing has become a widely used technique to study cellular diversity and function. However, accurately annotating cell types from single cell data has been a challenging task, as it requires extensive…

Genomics · Quantitative Biology 2023-04-07 Zehua Zeng , Hongwu Du

One of the most challenging aspects of medical image analysis is the lack of a high quantity of annotated data. This makes it difficult for deep learning algorithms to perform well due to a lack of variations in the input space. While…

Computer Vision and Pattern Recognition · Computer Science 2020-03-13 Soumyajyoti Dey , Soham Das , Swarnendu Ghosh , Shyamali Mitra , Sukanta Chakrabarty , Nibaran Das

In recent years, the field of single-cell data analysis has seen a marked advancement in the development of clustering methods. Despite advancements, most of these algorithms still concentrate on analyzing the provided single-cell matrix…

Machine Learning · Computer Science 2023-12-18 Dayu Hu , Ke Liang , Hao Yu , Xinwang Liu

Automatic analysis of spatio-temporal microscopy images is inevitable for state-of-the-art research in the life sciences. Recent developments in deep learning provide powerful tools for automatic analyses of such image data, but heavily…

Image and Video Processing · Electrical Eng. & Systems 2021-01-28 Dennis Bähr , Dennis Eschweiler , Anuk Bhattacharyya , Daniel Moreno-Andrés , Wolfram Antonin , Johannes Stegmaier

Image annotation is one essential prior step to enable data-driven algorithms. In medical imaging, having large and reliably annotated data sets is crucial to recognize various diseases robustly. However, annotator performance varies…

Image and Video Processing · Electrical Eng. & Systems 2022-11-23 Sonja Kunzmann , Mathias Öttl , Prathmesh Madhu , Felix Denzinger , Andreas Maier

Flow cytometry is a widespread single-cell measurement technology with a multitude of clinical and research applications. Interpretation of flow cytometry data is hard; the instrumentation is delicate and can not render absolute…

Applications · Statistics 2015-02-16 Jonas Wallin , Kerstin Johnsson , Magnus Fontes

High quality gene models are necessary to expand the molecular and genetic tools available for a target organism, but these are available for only a handful of model organisms that have undergone extensive curation and experimental…

Accurate classification of haematological cells is critical for diagnosing blood disorders, but presents significant challenges for machine automation owing to the complexity of cell morphology, heterogeneities of biological, pathological,…

Cell tracking remains a pivotal yet challenging task in biomedical research. The full potential of deep learning for this purpose is often untapped due to the limited availability of comprehensive and varied training data sets. In this…

Computer Vision and Pattern Recognition · Computer Science 2024-04-30 Moritz Sturm , Lorenzo Cerrone , Fred A. Hamprecht

Gene annotation addresses the problem of predicting unknown associations between gene and functions (e.g., biological processes) of a specific organism. Despite recent advances, the cost and time demanded by annotation procedures that rely…

Machine Learning · Computer Science 2022-05-02 Miguel Romero , Oscar Ramírez , Jorge Finke , Camilo Rocha
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