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In the complex landscape of hematologic samples such as peripheral blood or bone marrow derived from flow cytometry (FC) data, cell-level prediction presents profound challenges. This work explores injecting hierarchical prior knowledge…

Machine Learning · Computer Science 2024-07-30 Fatemeh Nassajian Mojarrad , Lorenzo Bini , Thomas Matthes , Stéphane Marchand-Maillet

A Bayesian feature allocation model (FAM) is presented for identifying cell subpopulations based on multiple samples of cell surface or intracellular marker expression level data obtained by cytometry by time of flight (CyTOF). Cell…

Applications · Statistics 2020-02-21 Arthur Lui , Juhee Lee , Peter F. Thall , May Daher , Katy Rezvani , Rafet Barar

Novel multimodal imaging methods are capable of generating extensive, super high resolution datasets for preclinical research. Yet, a massive lack of annotations prevents the broad use of deep learning to analyze such data. So far, existing…

Image and Video Processing · Electrical Eng. & Systems 2021-04-26 Izabela Horvath , Johannes C. Paetzold , Oliver Schoppe , Rami Al-Maskari , Ivan Ezhov , Suprosanna Shit , Hongwei Li , Ali Ertuerk , Bjoern H. Menze

Segmenting coronary arteries is challenging, as classic unsupervised methods fail to produce satisfactory results and modern supervised learning (deep learning) requires manual annotation which is often time-consuming and can some time be…

Image and Video Processing · Electrical Eng. & Systems 2019-07-29 Fei Yu , Jie Zhao , Yanjun Gong , Zhi Wang , Yuxi Li , Fan Yang , Bin Dong , Quanzheng Li , Li Zhang

Network models provide a powerful framework for analysing single-cell count data, facilitating the characterisation of cellular identities, disease mechanisms, and developmental trajectories. However, uncertainty modeling in unsupervised…

Genomics · Quantitative Biology 2026-04-27 Shanshan Ren , Thomas E. Bartlett , Lina Gerontogianni , Swati Chandna

The ocean is filled with phytoplankton that contribute as much photosynthesis as all land plants combined, making them vital to the carbon cycle and climate system. Recent advances in flow cytometry allow oceanographers to measure the…

Methodology · Statistics 2026-03-09 Yik Lun Kei , Qi Wang , Paul Parker , Francois Ribalet , Sangwon Hyun

Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to analyze gene expression at the resolution of individual cells, providing unprecedented insights into cellular heterogeneity and complex biological systems. This paper…

Other Quantitative Biology · Quantitative Biology 2024-06-11 Megha Patel , Nimish Magre , Himanshi Motwani , Nik Bear Brown

Mitosis nuclei count is one of the important indicators for the pathological diagnosis of breast cancer. The manual annotation needs experienced pathologists, which is very time-consuming and inefficient. With the development of deep…

Computer Vision and Pattern Recognition · Computer Science 2022-12-29 Huadeng Wang , Zhipeng Liu , Rushi Lan , Zhenbing Liu , Xiaonan Luo , Xipeng Pan , Bingbing Li

Biomedical research increasingly relies on 3D cell culture models and AI-based analysis can potentially facilitate a detailed and accurate feature extraction on a single-cell level. However, this requires for a precise segmentation of 3D…

Image and Video Processing · Electrical Eng. & Systems 2024-08-30 Roman Bruch , Mario Vitacolonna , Elina Nürnberg , Simeon Sauer , Rüdiger Rudolf , Markus Reischl

We introduce AttriGen, a novel framework for automated, fine-grained multi-attribute annotation in computer vision, with a particular focus on cell microscopy where multi-attribute classification remains underrepresented compared to…

Computer Vision and Pattern Recognition · Computer Science 2025-10-01 Walid Houmaidi , Youssef Sabiri , Fatima Zahra Iguenfer , Amine Abouaomar

Bone marrow cytology is required to make a hematological diagnosis, influencing critical clinical decision points in hematology. However, bone marrow cytology is tedious, limited to experienced reference centers and associated with high…

Image and Video Processing · Electrical Eng. & Systems 2021-07-09 Rohollah Moosavi Tayebi , Youqing Mu , Taher Dehkharghanian , Catherine Ross , Monalisa Sur , Ronan Foley , Hamid R. Tizhoosh , Clinton JV Campbell

In systems biomedicine, an experimenter encounters different potential sources of variation in data such as individual samples, multiple experimental conditions, and multi-variable network-level responses. In multiparametric cytometry,…

Many modern biological assays, including RNA sequencing, yield integer-valued counts that reflect the number of molecules detected. These measurements are often not at the desired resolution: while the unit of interest is typically a single…

Machine Learning · Computer Science 2026-03-06 Nic Fishman , Gokul Gowri , Tanush Kumar , Jiaqi Lu , Valentin de Bortoli , Jonathan S. Gootenberg , Omar Abudayyeh

We propose a novel method, scTree, for single-cell Tree Variational Autoencoders, extending a hierarchical clustering approach to single-cell RNA sequencing data. scTree corrects for batch effects while simultaneously learning a…

Machine Learning · Computer Science 2024-07-11 Moritz Vandenhirtz , Florian Barkmann , Laura Manduchi , Julia E. Vogt , Valentina Boeva

Identifying cell types and subtypes in routine histopathology is fundamental for understanding disease. Existing tile-based models capture nuclear detail but miss the broader tissue context that influences cell identity. Current human…

Computer Vision and Pattern Recognition · Computer Science 2025-11-20 Yinuo Xu , Yan Cui , Mingyao Li , Zhi Huang

Deep learning has achieved spectacular performance in image and speech recognition and synthesis. It outperforms other machine learning algorithms in problems where large amounts of data are available. In the area of measurement technology,…

Quantitative Methods · Quantitative Biology 2019-08-20 Yueqin Li , Ata Mahjoubfar , Claire Lifan Chen , Kayvan Reza Niazi , Li Pei , Bahram Jalali

The annotation of large scale histopathology image datasets remains a major bottleneck in developing robust deep learning models for clinically relevant tasks, such as mitotic figure classification. Folder-based annotation workflows are…

Many biological data analysis processes like Cytometry or Next Generation Sequencing (NGS) produce massive amounts of data which needs to be processed in batches for down-stream analysis. Such datasets are prone to technical variations due…

Machine Learning · Computer Science 2019-06-24 Uddeshya Upadhyay , Arjun Jain

Multi-class cell segmentation in high-resolution Giga-pixel whole slide images (WSI) is critical for various clinical applications. Training such an AI model typically requires labor-intensive pixel-wise manual annotation from experienced…

The rapid advancement of single-cell ATAC sequencing (scATAC-seq) technologies holds great promise for investigating the heterogeneity of epigenetic landscapes at the cellular level. The amplification process in scATAC-seq experiments often…

Genomics · Quantitative Biology 2024-08-28 Lei Huang , Lei Xiong , Na Sun , Zunpeng Liu , Ka-Chun Wong , Manolis Kellis