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Femtosecond-scale ultrafast imaging is an essential tool for visualizing ultrafast dynamics in molecular biology, physical chemistry, atomic physics, and fluid dynamics. Pump-probe imaging and a streak camera are the most widely used…

Optics · Physics 2020-03-18 Jungho Moon , Seok-Chan Yoon , Yong-sik Lim , Wonshik Choi

We present a new effective way for performance capture of deforming meshes with fine-scale time-varying surface detail from multi-view video. Our method builds up on coarse 4D surface reconstructions, as obtained with commonly used…

Computer Vision and Pattern Recognition · Computer Science 2016-02-08 Nadia Robertini , Edilson De Aguiar , Thomas Helten , Christian Theobalt

Medical Hyperspectral Imaging (MHSI) has emerged as a promising tool for enhanced disease diagnosis, particularly in computational pathology, offering rich spectral information that aids in identifying subtle biochemical properties of…

Computer Vision and Pattern Recognition · Computer Science 2025-07-10 Qing Zhang , Guoquan Pei , Yan Wang

Volumetric biomedical microscopy has the potential to increase the diagnostic information extracted from clinical tissue specimens and improve the diagnostic accuracy of both human pathologists and computational pathology models.…

Image and Video Processing · Electrical Eng. & Systems 2024-04-16 Cheng Jiang , Alexander Gedeon , Yiwei Lyu , Eric Landgraf , Yufeng Zhang , Xinhai Hou , Akhil Kondepudi , Asadur Chowdury , Honglak Lee , Todd Hollon

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

Super-resolving medical images can help physicians in providing more accurate diagnostics. In many situations, computed tomography (CT) or magnetic resonance imaging (MRI) techniques capture several scans (modes) during a single…

With the advent of microsphere assisted microscopy in 2011, this technique emerged as a simple and easy way to obtain optical super-resolution. Although the possible mechanisms of imaging by microspheres are debated in the literature, most…

Optics · Physics 2015-08-27 Navid Farahi

Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…

Optics · Physics 2017-08-02 Hsiou-Yuan Liu , Jingshan Zhong , Laura Waller

Different imaging modalities are used to extract the diverse information carried in an optical field. Two prominent modalities include bright field and phase contrast microscopy that can visualize the amplitude and phase features of a…

Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…

Ptychography is an enabling coherent diffraction imaging technique for both fundamental and applied sciences. Its applications in optical microscopy, however, fall short for its low imaging throughput and limited resolution. Here, we report…

The resolution of photoacoustic imaging deep inside scattering media is limited by the acoustic diffraction limit. In this work, taking inspiration from super-resolution imaging techniques developed to beat the optical diffraction limit, we…

Optics · Physics 2017-11-15 Sergey Vilov , Bastien Arnal , Emmanuel Bossy

Confocal microscopy is the standard approach for obtaining volumetric images of a sample with high axial and lateral resolution, especially when dealing with scattering samples. Unfortunately, a confocal microscope is quite expensive…

We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing…

Quantitative Methods · Quantitative Biology 2016-09-08 Gil Bub , Matthias Tecza , Michiel Helmes , Peter Lee , Peter Kohl

The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using the time-domain fingerprints from uniform nanoparticles. However, the optical diffraction limit compromises the decoding throughput and…

Optics · Physics 2022-08-09 Baolei Liu , Jiayan Liao , Yiliao Song , Jie Lu , Jiajia Zhou , Fan Wang

We report the development of a multichannel microscopy for whole-slide multiplane, multispectral, and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data…

Instrumentation and Detectors · Physics 2017-08-24 Jun Liao , Zhe Wang , Zibang Zhang , Zichao Bian , Kaikai Guo , Aparna Nambiar , Yutong Jiang , Shaowei Jiang , Jingang Zhong , Michael Choma , Guoan Zheng

Major biological discoveries have been made by interrogating living organisms with light. However, the limited penetration of unscattered photons within biological tissues severely limits the depth range covered by optical methods.…

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Absorption microscopy is a powerful technique, enabling the detection of single non- fluorescent molecules at room temperature. So far, the molecular absorption has been probed optically via the attenuation of a probing laser. The…

Applied Physics · Physics 2018-11-21 Miao-Hsuan Chien , Mario Brameshuber , Gerhard J. Schütz , Silvan Schmid

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

Optics · Physics 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked