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Confocal imaging and phase imaging are powerful tools in life science research and industrial inspection. To coherently link the two techniques with different depth resolutions, we introduce an optical frequency comb (OFC) to microscopy.…

Microscopes face a trade-off between spatial resolution, field-of-view, and frame rate -- improving one of these properties typically requires sacrificing the others, due to the limited spatiotemporal throughput of the sensor. To overcome…

One of the main characteristics of optical imaging systems is the spatial resolution, which is restricted by the diffraction limit to approximately half the wavelength of the incident light. Along with the recently developed classical…

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…

Many approaches to 3D image segmentation are based on hierarchical clustering of supervoxels into image regions. Here we describe a distributed algorithm capable of handling a tremendous number of supervoxels. The algorithm works…

Computer Vision and Pattern Recognition · Computer Science 2021-06-22 Ran Lu , Aleksandar Zlateski , H. Sebastian Seung

As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and…

Recent developments in image acquisition literature have miniaturized the confocal laser endomicroscopes to improve usability and flexibility of the apparatus in actual clinical settings. However, miniaturized devices collect less light and…

Image and Video Processing · Electrical Eng. & Systems 2019-07-24 Saeed Izadi , Darren Sutton , Ghassan Hamarneh

The thin and flexible nature of optical fibres often makes them the ideal technology to view biological processes in-vivo, but current microendoscopic approaches are limited in spatial resolution. Here, we demonstrate a new route to high…

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

High-resolution optical microscopy has transformed biological imaging, yet its resolution and contrast deteriorate with depth due to multiple light scattering. Conventional correction strategies typically approximate the medium as one or a…

The capability of focus control has been central to optical technologies that require both high temporal and spatial resolutions. However, existing varifocal lens schemes are commonly limited to the response time on the microsecond…

Super-resolution imaging with advanced optical systems has been revolutionizing technical analysis in various fields from biological to physical sciences. However, many objects are hidden by strongly scattering media such as rough wall…

Optics · Physics 2021-09-06 Dong Wang , Sujit Kumar Sahoo , Cuong Dang

Multi-spectral imaging, which simultaneously captures the spatial and spectral information of a scene, is widely used across diverse fields, including remote sensing, biomedical imaging, and agricultural monitoring. Here, we introduce a…

We present an ultra-thin hybrid imaging system based on an optical multimode fiber (MMF) and an optical fiber hydrophone that combines optical resolution photoacoustic and fluorescence microscopy. To control the illumination at the distal…

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Medical Physics · Physics 2025-09-22 Kyriakos Pentarakis , George Kakavelakis , Constantinos Petridis , George Themelis

Multispectral imaging is very beneficial in diverse applications, like healthcare and agriculture, since it can capture absorption bands of molecules in different spectral areas. A promising approach for multispectral snapshot imaging are…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Frank Sippel , Jürgen Seiler , André Kaup

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 {\mu}m axial and 0.4 {\mu}m lateral resolution maintained over a depth of 40 {\mu}m, while preserving the advantages of Fourier domain OCM. Our method uses…

Widefield microscopy methods applied to optically thick specimens are faced with reduced contrast due to spatial crosstalk, in which the signal at each point is the result of a superposition from neighboring points that are simultaneously…

Imaging through opaque, highly scattering walls is a long sought after capability with potential applications in a variety of fields. The use of wavefront shaping to compensate for scattering has brought a renewed interest as a potential…