Pixel multiplexing for high-speed multi-resolution fluorescence imaging
Quantitative Methods
2016-09-08 v1 Biological Physics
Tissues and Organs
Abstract
We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imaging calcium transients in heart cells at 250 Hz with a 10 Hz megapixel camera.
Keywords
Cite
@article{arxiv.0910.0789,
title = {Pixel multiplexing for high-speed multi-resolution fluorescence imaging},
author = {Gil Bub and Matthias Tecza and Michiel Helmes and Peter Lee and Peter Kohl},
journal= {arXiv preprint arXiv:0910.0789},
year = {2016}
}
Comments
3 pages, 2 figures, submitted to Nature Methods