English
Related papers

Related papers: Fast fluorescence lifetime imaging analysis via ex…

200 papers

Mammography is often used as the most common laboratory method for the detection of breast cancer, yet associated with the high cost and many side effects. Machine learning prediction as an alternative method has shown promising results.…

Image and Video Processing · Electrical Eng. & Systems 2019-10-31 Sanaz Mojrian , Gergo Pinter , Javad Hassannataj Joloudari , Imre Felde , Narjes Nabipour , Laszlo Nadai , Amir Mosavi

Extreme learning machine (ELM) as an emerging branch of shallow networks has shown its excellent generalization and fast learning speed. However, for blended data, the robustness of ELM is weak because its weights and biases of hidden nodes…

Machine Learning · Computer Science 2014-09-24 Bo Han , Bo He , Mengmeng Ma , Tingting Sun , Tianhong Yan , Amaury Lendasse

We consider the use of extreme learning machines (ELM) for computational partial differential equations (PDE). In ELM the hidden-layer coefficients in the neural network are assigned to random values generated on $[-R_m,R_m]$ and fixed,…

Computational Physics · Physics 2022-06-01 Suchuan Dong , Jielin Yang

The development of voltage-sensitive fluorescent probes suggests fluorescence lifetime as a promising readout for electrical activity in biological systems. Existing approaches fail to achieve the speed and sensitivity required for voltage…

Optics · Physics 2023-06-27 Adam J. Bowman , Cheng Huang , Mark J. Schnitzer , Mark A. Kasevich

The tracking method based on the extreme learning machine (ELM) is efficient and effective. ELM randomly generates input weights and biases in the hidden layer, and then calculates and computes the output weights by reducing the iterative…

Machine Learning · Computer Science 2018-07-27 Jing Zhang , Huibing Wang , Yonggong Ren

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…

Instrumentation and Detectors · Physics 2017-08-14 Egidijus Auksorius

Scanning Electron Microscopy (SEM) is pivotal in revealing intricate micro- and nanoscale features across various research fields. However, obtaining high-resolution SEM images presents challenges, including prolonged scanning durations and…

Image and Video Processing · Electrical Eng. & Systems 2024-10-08 Tom Reclik , Setareh Medghalchi , Philipp Schumacher , Maximilian Wollenweber , Talal Al-Samman , Sandra Korte-Kerzel , Ulrich Kerzel

Extreme Learning Machines (ELMs) have become a popular tool in the field of Artificial Intelligence due to their very high training speed and generalization capabilities. Another advantage is that they have a single hyper-parameter that…

Machine Learning · Computer Science 2019-12-05 Nicolás Nieto , Francisco Ibarrola , Victoria Peterson , Hugo Rufiner , Ruben Spies

Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…

Instrumentation and Detectors · Physics 2018-10-12 Matthias Eibl , Sebastian Karpf , Hubertus Hakert , Daniel Weng , Robert Huber

Live-cell imaging of multiple subcellular structures is essential for understanding subcellular dynamics. However, the conventional multi-color sequential fluorescence microscopy suffers from significant imaging delays and limited number of…

Subcellular Processes · Quantitative Biology 2025-01-13 Mingyang Chen , Luhong Jin , Xuwei Xuan , Defu Yang , Yun Cheng , Ju Zhang

The ability to estimate epistemic uncertainty is often crucial when deploying machine learning in the real world, but modern methods often produce overconfident, uncalibrated uncertainty predictions. A common approach to quantify epistemic…

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Multilayer Extreme Learning Machine (ML-ELM) and its variants have proven to be an effective technique for the classification of different natural signals such as audio, video, acoustic and images. In this paper, a Hybrid Multilayer Extreme…

Computer Vision and Pattern Recognition · Computer Science 2025-07-14 Rolando A. Hernandez-Hernandez , Adrian Rubio-Solis

Efficient and accurate joint representation of a collection of images, that belong to the same class, is a major research challenge for practical image set classification. Existing methods either make prior assumptions about the data…

Computer Vision and Pattern Recognition · Computer Science 2015-04-02 Muhammad Uzair , Faisal Shafait , Bernard Ghanem , Ajmal Mian

We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet…

Extreme learning machine (ELM) is a network model that arbitrarily initializes the first hidden layer and can be computed speedily. In order to improve the classification performance of ELM, a $\ell_2$ and $\ell_{0.5}$ regularization ELM…

Optimization and Control · Mathematics 2023-01-05 Liangjuan Zhou , Wei Miao

Medical image segmentation is a relevant problem, with deep learning being an exponent. However, the necessity of a high volume of fully annotated images for training massive models can be a problem, especially for applications whose images…

Computer Vision and Pattern Recognition · Computer Science 2024-06-06 Matheus A. Cerqueira , Flávia Sprenger , Bernardo C. A. Teixeira , Alexandre X. Falcão

The recent development of deep learning large models in medicine shows remarkable performance in medical image analysis and diagnosis, but their large number of parameters causes memory and inference latency challenges. Knowledge…

Computer Vision and Pattern Recognition · Computer Science 2024-09-19 Shaojie Li , Zhaoshuo Diao