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Optoplasmonic methods capable of single protein detection so far rely on analyte immobilization in order to facilitate detection [1-6]. These detection schemes, even if they facilitate transient single-molecule detection [7,8] via…

Optics · Physics 2022-01-21 M. D. Baaske , N. Asgari , D. Punj , M. Orrit

The measurement of the spatio-temporal correlations of light provides an interesting tool to overcome the traditional limitations of standard imaging, such as the strong trade-off between spatial resolution and depth of field. In…

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…

Scanning probe microscopy (SPM) is ubiquitous in nanoscale science allowing the observation of features in real space down to the angstrom resolution. The scanning nature of SPM, wherein a sharp tip rasters the surface during which a…

Instrumentation and Detectors · Physics 2024-11-20 Maxime Le Ster , Sławomir Pawłowski , Iaroslav Lutsyk , Paweł Janusz Kowalczyk

Spatial resolution is a fundamental parameter in structural sciences. In crystallography, the resolution is determined from the detection limit of high-angle diffraction in reciprocal space. In electron microscopy, correlation in the…

Scanning tunnelling microscopy (STM) is a powerful technique for imaging surfaces with atomic resolution, providing insight into physical and chemical processes at the level of single atoms and molecules. A regular task of STM image…

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

We introduce a new algorithm to solve a regularized spatial-spectral image estimation problem. Our approach is based on the linearized alternating directions method of multipliers (LADMM), which is a variation of the popular ADMM algorithm.…

Signal Processing · Electrical Eng. & Systems 2025-02-25 Yunsong Liu , Debdut Mandal , Congyu Liao , Kawin Setsompop , Justin P. Haldar

Soliton molecules (SMs) are fundamentally important modes in nonlinear optical systems. It is a challenge to experimentally produce SMs with a required temporal separation in mode-locked fiber lasers. Here, we propose and realize an…

Optics · Physics 2022-02-09 Shilong Liu , Yudong Cui , Ebrahim Karimi , Boris A. Malomed

A data movie of stochastic optical localization nanoscopy contains spatial and temporal correlations, both providing information of emitter locations. The majority of localization algorithms in the literature estimate emitter locations by…

Image and Video Processing · Electrical Eng. & Systems 2020-07-21 Yi Sun

Spatial Fluorescence Cross Correlation Spectroscopy is a rarely investigated version of Fluorescence Correlation Spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported…

Optics · Physics 2008-06-13 Yoann Blancquaert , Jie Gao , Jacques Derouard , Antoine Delon

As the ultimate miniaturization of semiconductor devices approaches, it is imperative that the effects of single dopants be clarified. Beyond providing insight into functions and limitations of conventional devices, such information enables…

Mesoscale and Nanoscale Physics · Physics 2016-10-27 Mohammad Rashidi , Jacob Burgess , Marco Taucer , Roshan Achal , Jason L. Pitters , Sebastian Loth , Robert A. Wolkow

It is shown that quantitative information on spatial correlations in a system of polarizable particles can be extracted directly from its experimentally measurable optical spectra. For a collection of metallic nanoparticles (NPs), it is…

Mesoscale and Nanoscale Physics · Physics 2015-06-15 R. M. S. Pereira , P. Pereira , G. Smirnov , M. I. Vasilevskiy

Schemes for X-ray imaging single protein molecules using new x-ray sources, like x-ray free electron lasers (XFELs), require processing many frames of data that are obtained by taking temporally short snapshots of identical molecules, each…

Data Analysis, Statistics and Probability · Physics 2015-06-17 Kartik Ayyer , Hugh T. Philipp , Mark W. Tate , Veit Elser , Sol M. Gruner

Optically read out gaseous detectors are used in track reconstruction and imaging applications requiring high granularity images. Among resolution-determining factors, the amplification stage plays a crucial role and optimisations of…

Instrumentation and Detectors · Physics 2024-07-23 A. Cools , E. Ferrer-Ribas , T. Papaevangelou , E. C. Pollacco , M. Lisowska , F. M. Brunbauer , E. Oliveri , F. J. Iguaz

Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…

Optics · Physics 2020-08-05 Daniel Böning , Franz-Ferdinand Wieser , Vahid Sandoghdar

Multispectral imaging plays an important role in many applications from astronomical imaging, earth observation to biomedical imaging. However, the current technologies are complex with multiple alignment-sensitive components, predetermined…

Optics · Physics 2021-09-17 Sujit Kumar Sahoo , Dongliang Tang , Cuong Dang

In this paper, we have established the couple system of a spectroscope, CCD and computer and proposed a method of the rapid measurement on spectral characteristics such as central wavelengths, relative intensities, sensitivity lines and the…

Instrumentation and Detectors · Physics 2015-12-03 Chol-Sun Kim , Chol-Su Kim , Song-Jin Im

We report on image processing techniques and experimental procedures to determine the lattice-site positions of single atoms in an optical lattice with high reliability, even for limited acquisition time or optical resolution. Determining…

Semi monolithic detector designs with a series of stacked thin monolithic scintillator plates and side readout are an attractive approach for potentially achieving very high performance in a positron emission tomography (PET) scanner. In…

Instrumentation and Detectors · Physics 2025-02-12 Shiv K. Subedi , Simon R. Cherry , Yi Qiang , Peng Peng