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Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…

Quantitative Methods · Quantitative Biology 2017-10-24 C. Chen , P. Paul-Gilloteaux , T. Vignaud , J. Salamero , F. Waharte

Structured illumination in Single Molecule Localization Microscopy provides new information on the position of molecules and thus improves the localization precision compared to standard localization methods. Here, we used a time-shifted…

Recent work has shown impressive localization performance using only images of ground textures taken with a downward facing monocular camera. This provides a reliable navigation method that is robust to feature sparse environments and…

Robotics · Computer Science 2023-03-13 Kyle M. Hart , Brendan Englot , Ryan P. O'Shea , John D. Kelly , David Martinez

We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and…

Biomolecules · Quantitative Biology 2015-06-24 Zhaokun Zhou , Helen Miller , Adam J. M. Wollman , Mark C. Leake

We demonstrate a single-photon sensitive spectrometer in the visible range, which allows us to perform time-resolved and multi-photon spectral correlation measurements. It is based on a monochromator composed of two gratings, collimation…

Assessing the quality of parameter estimates for models describing the motion of single molecules in cellular environments is an important problem in fluorescence microscopy. We consider the fundamental data model, where molecules emit…

Quantitative Methods · Quantitative Biology 2021-09-15 A. Marie d'Avigneau , S. S. Singh , R. J. Ober

The measurement rate of cameras that take spatially multiplexed measurements by using spatial light modulators (SLM) is often limited by the switching speed of the SLMs. This is especially true for single-pixel cameras where the…

Computer Vision and Pattern Recognition · Computer Science 2015-03-17 Jian Wang , Mohit Gupta , Aswin C. Sankaranarayanan

Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

Ultrasound localization microscopy (ULM) refers to a promising medical imaging modality that systematically leverages the advantages of contrast-enhanced ultrasound (CEUS) to surpass the diffraction barrier and delineate the microvascular…

Image and Video Processing · Electrical Eng. & Systems 2022-09-22 Md Ashikuzzaman , Brandon Helfield , Hassan Rivaz

Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

Monocular simultaneous localization and mapping (SLAM) algorithms estimate drone poses and build a 3D map using a single camera. Current algorithms include sparse methods that lack detailed geometry, while learning-driven approaches produce…

Robotics · Computer Science 2025-11-25 Jeryes Danial , Yosi Ben Asher , Itzik Klein

The complementary fusion of light detection and ranging (LiDAR) data and image data is a promising but challenging task for generating high-precision and high-density point clouds. This study proposes an innovative LiDAR-guided stereo…

Computer Vision and Pattern Recognition · Computer Science 2022-02-25 Yongjun Zhang , Siyuan Zou , Xinyi Liu , Xu Huang , Yi Wan , Yongxiang Yao

Precise measurement of multi-parameters of ultrafast lasers is vital both in scientific investigations and technical applications, such as, optical field manipulation, pulse shaping, sample characteristics test, and biomedical imaging.…

Single-shot diffraction imaging of isolated nanosized particles has seen remarkable success in recent years, yielding in-situ measurements with ultra-high spatial and temporal resolution. The progress of high-repetition-rate sources for…

Atomic and Molecular Clusters · Physics 2022-08-26 Julian Zimmermann , Fabien Beguet , Daniel Guthruf , Bruno Langbehn , Daniela Rupp

We propose spatial polarization multiplexing (SPM) for joint sensing of shape and reflectance of a static or dynamic deformable object, which is also invisible to the naked eye. Past structured-light methods are limited to shape acquisition…

Computer Vision and Pattern Recognition · Computer Science 2026-01-07 Tomoki Ichikawa , Ryo Kawahara , Ko Nishino

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

The log-ratio (LR) operator has been widely employed to generate the difference image for synthetic aperture radar (SAR) image change detection. However, the difference image generated by this pixel-wise operator can be subject to SAR…

Computer Vision and Pattern Recognition · Computer Science 2020-02-19 Rongfang Wang , Jia-Wei Chen , Yule Wang , Licheng Jiao , Mi Wang

We prove that it is impossible to distinguish two spatially coinciding fluorescent molecules from a single rotating molecule using polarization-sensitive imaging, even if one modulates the polarization of the illumination or the detection…

Optics · Physics 2025-03-11 Yiyang Chen , Yuanxin Qiu , Matthew D. Lew

We have developed a general approach for the calculation of the single molecule polarization correlation function C(t), which delivers a correlation of the emission dichroisms at time 0 and t. The approach is model independent and valid for…

Chemical Physics · Physics 2007-05-23 M. F. Gelin , D. S. Kosov