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Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…

Biomolecules · Quantitative Biology 2011-07-12 Shannon Kian Zareh , Michael C. DeSantis , Jonathan Kessler , Je-Luen Li , Y. M. Wang

To be able to resolve molecular-clusters it is crucial to access vital informations (such as, molecule density and cluster-size) that are key to understand disease progression and the underlying mechanism. Traditional single-molecule…

Quantitative Methods · Quantitative Biology 2021-01-27 Partha Pratim Mondal

Transient Photoluminescence Microscopy (TPLM) allows for the direct visualization of carrier transport in semiconductor materials with sub nanosecond and few nanometer resolution. The technique is based on measuring changes in the spatial…

The inherent non-linearity of intensity correlation functions can be used to spatially distinguish identical emitters beyond the diffraction limit, as achieved, for example, in Super-Resolution Optical Fluctuation Imaging (SOFI). Here, we…

Optics · Physics 2024-12-18 Yifan Chen , Chieh Tsao , Hendrik Utzat

Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…

Optics · Physics 2024-07-11 Hanzhe Wu , Danni Chen , YiHong Jiand Gan Xiang , Heng Li , Bin Yu , JunLe Qu

Split-pulse x-ray photon correlation spectroscopy has been proposed as one of the unique capabilities made possible with the x-ray free electron lasers. It enables characterization of atomic scale structural dynamics that dictates the…

Instrumentation and Detectors · Physics 2020-05-06 Yanwen Sun , Mike Dunne , Paul Fuoss , Taito Osaka , Aymeric Robert , Mark Sutton , Makina Yabashi , Diling Zhu

Optical solitary waves (solitons) that interact in a nonlinear system can bind and form a structure similar to a molecule. The rich dynamics of this process have created a demand for rapid spectral characterization to deepen the…

Optics · Physics 2023-06-21 Lukasz A. Sterczewski , Jaroslaw Sotor

Spectral diffusion is a result of random spectral jumps of a narrow line as a result of a fluctuating environment. It is an important issue in spectroscopy, because the observed spectral broadening prevents access to the intrinsic line…

Super Resolution (SR) plays a critical role in computer vision, particularly in medical imaging, where hardware and acquisition time constraints often result in low spatial and temporal resolution. While diffusion models have been applied…

Image and Video Processing · Electrical Eng. & Systems 2024-11-01 Vishal Dubey

We present a theory for analyzing residence times of single molecules in a fixed detection area of a scanning tunneling microscope (STM). The approach is developed for one-dimensional molecule diffusion and can be extended to two dimensions…

Mesoscale and Nanoscale Physics · Physics 2022-08-23 Christoph Schiel , Philipp Rahe , Philipp Maass

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

We present an analytical method to quantify clustering in super-resolution localization images of static surfaces in two dimensions. The method also describes how over-counting of labeled molecules contributes to apparent self-clustering…

Biological Physics · Physics 2012-03-13 Sarah Veatch , Benjamin Machta , Sarah Shelby , Ethan Chiang , David Holowka , Barbara Baird

The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…

We present fast parallel rearrangement of single atoms in optical tweezers into arbitrary geometries by updating holograms displayed by an ultra fast spatial light modulator. Using linear interpolation of the tweezer position and the…

There are many image fusion methods that can be used to produce high-resolution mutlispectral images from a high-resolution panchromatic (PAN) image and low-resolution multispectral (MS) of remote sensed images. This paper attempts to…

Computer Vision and Pattern Recognition · Computer Science 2011-08-17 Firouz Abdullah Al-Wassai , N. V. Kalyankar , Ali A. Al-Zaky

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

Lensless imaging is a popular research field for the advantages of small size, wide field-of-view and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors and…

In the maintenance of optical fiber networks, there is a growing demand for high-precision measurement of optical loss distribution and fault locations over long distances. In this study, we propose an OCDR method incorporating periodic…

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…

Optics · Physics 2013-09-02 Anthony Barsic , Ginni Grover , Rafael Piestun

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…