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The absence of robust segmentation frameworks for noisy liquid phase transmission electron microscopy (LPTEM) videos prevents reliable extraction of particle trajectories, creating a major barrier to quantitative analysis and to connecting…

Computer Vision and Pattern Recognition · Computer Science 2025-11-06 Alexander Wang , Max Xu , Risha Goel , Zain Shabeeb , Isabel Panicker , Vida Jamali

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the…

Computational Physics · Physics 2018-03-20 Amit Singer

The nanoparticle size and distribution information in the SEM images of silicon crystals are generally counted by manual methods. The realization of automatic machine recognition is significant in materials science. This paper proposed a…

Computer Vision and Pattern Recognition · Computer Science 2022-06-07 Ruiling Xiao , Jiayang Niu

Regular monitoring of the primary particles and purity profiles of a drug product during development and manufacturing processes is essential for manufacturers to avoid product variability and contamination. Transmission electron microscopy…

Computer Vision and Pattern Recognition · Computer Science 2023-11-10 Olivier Rukundo , Andrea Behanova , Riccardo De Feo , Seppo Ronkko , Joni Oja , Jussi Tohka

Structural Health Monitoring (SHM) is a sustainable and essential approach for infrastructure maintenance, enabling the early detection of structural defects. Leveraging computer vision (CV) methods for automated infrastructure monitoring…

Computer Vision and Pattern Recognition · Computer Science 2025-03-12 Yingchu Wang , Ji He , Shijie Yu

A growing need exists for efficient and accurate methods for detecting defects in semiconductor materials and devices. These defects can have a detrimental impact on the efficiency of the manufacturing process, because they cause critical…

Computer Vision and Pattern Recognition · Computer Science 2023-08-21 Thibault Lechien , Enrique Dehaerne , Bappaditya Dey , Victor Blanco , Sandip Halder , Stefan De Gendt , Wannes Meert

High-resolution structure determination by cryo-electron microscopy (cryo-EM) requires the accurate fitting of an atomic model into an experimental density map. Traditional refinement pipelines such as Phenix.real_space_refine and Rosetta…

Biomolecules · Quantitative Biology 2026-03-10 Fuyao Huang , Xiaozhu Yu , Kui Xu , Qiangfeng Cliff Zhang

This paper proposes a simple, automatic and efficient clustering algorithm, namely, Automatic Merging for Optimal Clusters (AMOC) which aims to generate nearly optimal clusters for the given datasets automatically. The AMOC is an extension…

Computer Vision and Pattern Recognition · Computer Science 2011-09-07 K. Karteeka Pavan , Allam Appa Rao , A. V. Dattatreya Rao

Cryo-electron microscopy (EM) single particle reconstruction is an entirely general technique for 3D structure determination of macromolecular complexes. However, because the images are taken at low electron dose, it is extremely hard to…

Computer Vision and Pattern Recognition · Computer Science 2019-04-17 Yifeng Fan , Zhizhen Zhao

Molecular electronics studies have advanced from early, simple single-molecule experiments at cryogenic temperatures to complex and multifunctional molecules under ambient conditions. However, room-temperature environments increase the risk…

Mesoscale and Nanoscale Physics · Physics 2025-06-12 Guillem Pellicer , Carlos Sabater

The detection of cracks is a crucial task in monitoring structural health and ensuring structural safety. The manual process of crack detection is time-consuming and subjective to the inspectors. Several researchers have tried tackling this…

Computer Vision and Pattern Recognition · Computer Science 2022-08-30 Shreyas Kulkarni , Shreyas Singh , Dhananjay Balakrishnan , Siddharth Sharma , Saipraneeth Devunuri , Sai Chowdeswara Rao Korlapati

Determining the three-dimensional structure of proteins and protein complexes at atomic resolution is a fundamental task in structural biology. Over the last decade, remarkable progress has been made using "single particle" cryo-electron…

Numerical Analysis · Mathematics 2017-06-05 Alex Barnett , Leslie Greengard , Andras Pataki , Marina Spivak

Cryo-electron tomography (cryo-ET) has emerged as a powerful technique for imaging macromolecular complexes in their near-native states. However, the localization of 3D particles in cellular environments still presents a significant…

Computer Vision and Pattern Recognition · Computer Science 2025-05-27 Gokul Adethya , Bhanu Pratyush Mantha , Tianyang Wang , Xingjian Li , Min Xu

Accurate characterization of carbon nanotube morphologies in electron microscopy images is vital for exposure assessment and toxicological studies, yet current workflows rely on slow, subjective manual segmentation. This work presents a…

Computer Vision and Pattern Recognition · Computer Science 2026-02-06 Sanjay Pradeep , Chen Wang , Matthew M. Dahm , Jeff D. Eldredge , Candace S. J. Tsai

Single particle cryo-electron microscopy (EM) is an increasingly popular method for determining the 3-D structure of macromolecules from noisy 2-D images of single macromolecules whose orientations and positions are random and unknown. One…

Computer Vision and Pattern Recognition · Computer Science 2017-04-11 Roy R. Lederman , Amit Singer

Macromolecules change their shape (conformation) in the process of carrying out their functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of macromolecules (single particles) is a powerful and general…

Biomolecules · Quantitative Biology 2022-11-22 Bogdan Toader , Fred J. Sigworth , Roy R. Lederman

Single particle cryogenic electron microscopy (cryo-EM) is an imaging technique capable of recovering the high-resolution 3-D structure of biological macromolecules from many noisy and randomly oriented projection images. One notable…

Biomolecules · Quantitative Biology 2024-01-30 Andy Zhang , Oscar Mickelin , Joe Kileel , Eric J. Verbeke , Nicholas F. Marshall , Marc Aurèle Gilles , Amit Singer

In single-particle cryo-electron microscopy (cryo-EM), K-means clustering algorithm is widely used in unsupervised 2D classification of projection images of biological macromolecules. 3D ab initio reconstruction requires accurate…

Quantitative Methods · Quantitative Biology 2017-01-03 Yaofang Xu , Jiayi Wu , Chang-Cheng Yin , Youdong Mao

Cryo-electron microscopy (cryo-EM) has revolutionized experimental protein structure determination. Despite advances in high resolution reconstruction, a majority of cryo-EM experiments provide either a single state of the studied…