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Chromosome analysis and identification from metaphase images is a critical part of cytogenetics based medical diagnosis. It is mainly used for identifying constitutional, prenatal and acquired abnormalities in the diagnosis of genetic…

Computer Vision and Pattern Recognition · Computer Science 2022-09-13 Amritha S Pallavoor , Prajwal A , Sundareshan TS , Sreekanth K Pallavoor

We presented a new 3D refinement method for Cryo-EM single particle analysis which can improve the resolution of final electron density map in this paper. We proposed to enforce both sparsity and smoothness to improve the regularity of…

Image and Video Processing · Electrical Eng. & Systems 2020-03-12 Zhenwei Luo

We present and rigorously analyze the behavior of a distributed, stochastic algorithm for separation and integration in self-organizing particle systems, an abstraction of programmable matter. Such systems are composed of individual…

Distributed, Parallel, and Cluster Computing · Computer Science 2019-06-06 Sarah Cannon , Joshua J. Daymude , Cem Gokmen , Dana Randall , Andréa W. Richa

In high-background or calibration measurements with cryogenic particle detectors, a significant share of the exposure is lost due to pile-up of recoil events. We propose a method for the separation of pile-up events with an LSTM neural…

Instrumentation and Detectors · Physics 2021-12-14 Felix Wagner

Single particle electron cryomicroscopy (cryo-EM) allows for structures of proteins and protein complexes to be determined from images of non-crystalline specimens. Cryo-EM data analysis requires electron microscope images of randomly…

Instrumentation and Detectors · Physics 2015-11-23 Jianhua Zhao , Marcus A. Brubaker , Samir Benlekbir , John L. Rubinstein

Nuclear instance segmentation and classification provide critical quantitative foundations for digital pathology diagnosis. With the advent of the foundational Segment Anything Model (SAM), the accuracy and efficiency of nuclear…

Image and Video Processing · Electrical Eng. & Systems 2025-04-04 Liying Xu , Hongliang He , Wei Han , Hanbin Huang , Siwei Feng , Guohong Fu

We introduce DiffFit, a differentiable algorithm for fitting protein atomistic structures into an experimental reconstructed Cryo-Electron Microscopy (cryo-EM) volume map. In structural biology, this process is necessary to…

Quantitative Methods · Quantitative Biology 2025-01-09 Deng Luo , Zainab Alsuwaykit , Dawar Khan , Ondřej Strnad , Tobias Isenberg , Ivan Viola

Non-destructive testing (NDT) is essential in ceramic manufacturing to ensure the quality of components without compromising their integrity. In this context, Optical Coherence Tomography (OCT) enables high-resolution internal imaging,…

Cryo-electron microscopy (cryo-EM), the subject of the 2017 Nobel Prize in Chemistry, is a technology for determining the 3-D structure of macromolecules from many noisy 2-D projections of instances of these macromolecules, whose…

Computer Vision and Pattern Recognition · Computer Science 2020-04-22 Roy R. Lederman , Joakim Andén , Amit Singer

A fundamental task in human chromosome analysis is chromosome segmentation. Segmentation plays an important role in chromosome karyotyping. The first step in segmentation is to remove intrusive objects such as stain debris and other noises.…

Computer Vision and Pattern Recognition · Computer Science 2014-09-02 Shervin Minaee , Mehran Fotouhi , Babak Hossein Khalaj

Medical image segmentation is vital for clinical diagnosis, yet current deep learning methods often demand extensive expert effort, i.e., either through annotating large training datasets or providing prompts at inference time for each new…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Xingjian Li , Qifeng Wu , Adithya S. Ubaradka , Yiran Ding , Colleen Que , Runmin Jiang , Jianhua Xing , Tianyang Wang , Min Xu

Cryo-electron microscopy (cryo-EM) has become a tool of fundamental importance in structural biology, helping us understand the basic building blocks of life. The algorithmic challenge of cryo-EM is to jointly estimate the unknown 3D poses…

Computer Vision and Pattern Recognition · Computer Science 2022-09-01 Axel Levy , Frédéric Poitevin , Julien Martel , Youssef Nashed , Ariana Peck , Nina Miolane , Daniel Ratner , Mike Dunne , Gordon Wetzstein

The Cryo-EM 3D particle reconstruction is essential for identifying protein and uncover the biological mechanism of the macro-molecules. In this paper, we use Kam method for reconstruction. Kam method is \textit{ab-initio}, and it assumes…

Quantitative Methods · Quantitative Biology 2019-06-21 Yin Xian

(abridged) We develop an algorithm for estimating parameters of a distribution sampled with contamination, employing a statistical technique known as ``expectation maximization'' (EM). Given models for both member and contaminant…

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a…

Quantitative Methods · Quantitative Biology 2024-03-14 Jeremy J. Axelrod , Jessie T. Zhang , Petar N. Petrov , Robert M. Glaeser , Holger Mueller

Constructing of molecular structural models from Cryo-Electron Microscopy (Cryo-EM) density volumes is the critical last step of structure determination by Cryo-EM technologies. Methods have evolved from manual construction by structural…

Machine Learning · Computer Science 2019-02-13 Kui Xu , Zhe Wang , Jiangping Shi , Hongsheng Li , Qiangfeng Cliff Zhang

To assist researchers to identify Environmental Microorganisms (EMs) effectively, a Multiscale CNN-CRF (MSCC) framework for the EM image segmentation is proposed in this paper. There are two parts in this framework: The first is a novel…

Computer Vision and Pattern Recognition · Computer Science 2021-02-25 Jinghua Zhang , Chen Li , Frank Kulwa , Xin Zhao , Changhao Sun , Zihan Li , Tao Jiang , Hong Li , Shouliang Qi

The in vitro clonogenic assay is a technique to study the ability of a cell to form a colony in a culture dish. By optical imaging, dishes with stained colonies can be scanned and assessed digitally. Identification, segmentation and…

Computer Vision and Pattern Recognition · Computer Science 2022-02-15 Delmon Arous , Stefan Schrunner , Ingunn Hanson , Nina F. J. Edin , Eirik Malinen

Automated experiments in scanning transmission electron microscopy (STEM) require rapid image segmentation to optimize data representation for human interpretation, decision-making, site-selective spectroscopies, and atomic manipulation.…

Materials Science · Physics 2024-09-23 Kamyar Barakati , Utkarsh Pratiush , Austin C. Houston , Gerd Duscher , Sergei V. Kalinin

The development of robust, real-time optical methods for the detection and tracking of particles in complex multiple scattering media is a problem of practical importance in a number of fields, including environmental monitoring, air…

Optics · Physics 2025-02-04 Mohammadrasoul Taghavi , Edwin A. Marengo
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