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Our work aims at using quantitative imaging tools to complement the limitation of noise encountered by high resolution fluorescence microscopy methods. Several cycles of fluorophore activation, imaging and deactivation produce a sequence of…

Quantitative Methods · Quantitative Biology 2012-02-03 Juliane Liepe , Federico Felizzi , Agata Pernus , Maria Hanulova

The combination of microfluidic technology and optical fluids characterization techniques has been recently applied to produce lab-on-a-chip systems. In the present work, bringing together the imaging technique called photoacoustic imaging…

The photothermal heterodyne imaging technique enabled studies of individual weakly absorbing nano-objects in various environments. It uses a photo-induced change in the refractive index of the environment. Taking advantage of the dramatic…

Mid-infrared photothermal microscopy is a highly promising imaging technique that enables spatially resolved vibrational fingerprinting. The combination of infrared induced heating with optical readout at visible wavelengths provides…

Optics · Physics 2025-08-05 Dennis van de Lockand , Daan Wolters , Matz Liebel

Presented in this paper are measurements of an optomechanical device in which various acoustic modes of a sample of superfluid helium couple to a fiber-based optical cavity. In contrast with recent work on the paraxial acoustic mode…

We show that the modulation of the phases of the laser beams of ultra-short pulses leads to modulation of the two photon fluorescence intensity. The phase modulation technique when used in multi-photon microscopy can improve the signal to…

Optics · Physics 2015-03-12 Khadga Jung Karki , Mohammed Abdellah , Tonu Pullerits

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

Optics · Physics 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

Optics · Physics 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

Photoacoustic microscopy is becoming an important tool for the biomedical research. It has been widely used in biological researches, such as structural imaging of vasculature, brain structural and functional imaging, and tumor detection.…

Image and Video Processing · Electrical Eng. & Systems 2020-09-09 Xianlin Song , Jianshuang Wei , Lingfang Song

Structured illumination enables the tailoring of an imaging device's optical transfer function to enhance resolution. We propose the incorporation of a temporal periodic modulation, specifically a rotating mask, to encode multiple transfer…

Optics · Physics 2023-06-27 Guillaume Noetinger , Fabrice Lemoult , Sébastien M. Popoff

Photoacoustic imaging has emerged in the past decades. Compared with the traditional medical imaging mode, it has better imaging performance and has great development potential in the field of biological imaging. In traditional…

Medical Physics · Physics 2020-09-03 Xianlin Song , Jianshuang Wei , Jianmin Xiong , Lingfang Song

This paper is devoted to the study of photothermal transformations in multilayered structures. As a modelled sample, porous silicon with a periodic distribution of the porosity was chosen. The spatial distribution of the optical properties…

Materials Science · Physics 2019-07-11 K. Dubyk , L. Chepela , P. Lishchuk , A. Belarouci , D. Lacroix , M. Isaiev

Major biological discoveries have been made by interrogating living organisms with light. However, the limited penetration of unscattered photons within biological tissues severely limits the depth range covered by optical methods.…

Acousto-optic devices utilize the overlap of acoustic and optical fields to facilitate photon-phonon interactions. For tightly confined optical and acoustic fields, such as the sub-wavelength scales achievable in integrated devices, this…

Optics · Physics 2021-01-21 Nathan Dostart , Miloš Popović

Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy,…

Optics · Physics 2024-10-11 Aymerick Bazin , Amaury Badon

Photoacoustic detection is a sensitive method for measurement of light-absorbing particles directly in the aerosol phase. In this article, we demonstrate a new sensitive technique for photoacoustic aerosol absorption measurements using a…

Instrumentation and Detectors · Physics 2021-06-24 Juho Karhu , Joel Kuula , Aki Virkkula , Hilkka Timonen , Markku Vainio , Tuomas Hieta

Photoacoustic imaging is the leading technique for deep tissue optical imaging, allowing single-shot imaging at depths. However, its resolution may be limited by acoustic aberrations, caused by natural unknown heterogeneities in the tissue…

Optics · Physics 2026-05-01 Yevgeny Slobodkin , Ori Katz

The back-focal plane (BFP) of a high-numerical aperture objective contains the fluoro-phore radiation pattern, which encodes information about the axial fluorophore position, molecular orientation and the local refractive index of the…

Optical micro-spectroscopy is an invaluable tool for studying and characterizing samples ranging from classical semiconductors to low-dimensional materials and heterostructures. To date, most implementations are based on point-scanning…

The research focuses on the introduction and validation of the lock-in photoacoustics of condensed systems in the presence of two simultaneous probing beams. The experiments, performed on three model systems: a perfect absorber - carbon…

Applied Physics · Physics 2025-02-06 Pawel Rochowski