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Intensity correlation microscopy (ICM), which is prominently known through antibunching microscopy or super-resolution optical fluctuation imaging (SOFI), provides superresolution through a correlation analysis of antibunching of…

Optics · Physics 2018-10-08 Anton Classen , Joachim von Zanthier , Girish S. Agarwal

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure…

Noise is a significant challenge in imaging. Conventional intensity-based techniques mitigate noise through various filtering methods, but they often require prior knowledge of noise characteristics and struggle, especially under low-light…

Optics · Physics 2026-05-12 Pranay Mohta , Keval Moliya , Aniket Nag , Shaurya Aarav , Anand Kumar Jha

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

We present an algorithm that uses the distribution of photon arrival times to distinguish speckles from incoherent sources, like planets and disks, in high contrast images. Using simulated data, we show that our approach can overcome the…

Instrumentation and Methods for Astrophysics · Physics 2019-10-09 Alex B. Walter , Clinton Bockstiegel , Timothy D. Brandt , Benjamin A. Mazin

Imaging of rapidly occurring events in biology requires high temporal resolution. In this review article, I have tried to investigate an approximate estimate to quantify temporal resolution limit of a fluorescence imaging system. It was…

Medical Physics · Physics 2014-07-25 Partha Pratim Mondal

Single-molecule fluorescence techniques are essential for investigating the molecular mechanisms in biological processes. However, achieving sub-millisecond temporal resolution to monitor fast molecular dynamics remains a significant…

Optics · Physics 2023-03-02 Sunny Tiwari , Prithu Roy , Jean-Benoît Claude , Jérôme Wenger

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

Data Structures and Algorithms · Computer Science 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song

We numerically investigate the role of quantum fluctuations in superresolution of optical objects. First, we confirm that when quantum fluctuations are not taken into account, one can easily improve the resolution by one order of magnitude…

Quantum Physics · Physics 2007-05-23 Vladislav N. Beskrovnyy , Mikhail I. Kolobov

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

We realize a scanning probe microscope using single trapped $^{87}$Rb atoms to measure optical fields with subwavelength spatial resolution. Our microscope operates by detecting fluorescence from a single atom driven by near-resonant light…

Atomic Physics · Physics 2022-03-02 Emma Deist , Justin A. Gerber , Yue-Hui Lu , Johannes Zeiher , Dan M. Stamper-Kurn

Interferometers are widely used in imaging technologies to achieve enhanced spatial resolution, but require that the incoming photons be indistinguishable. In previous work, we built and analyzed color erasure detectors which expand the…

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Resolution enhancements are often desired in imaging applications where high-resolution sensor arrays are difficult to obtain. Many computational imaging methods have been proposed to encode high-resolution scene information on…

Image and Video Processing · Electrical Eng. & Systems 2018-10-29 Kevin Beale , Jianbo Chen , Kevin F. Kelly , Justin Romberg

Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…

Biological Physics · Physics 2018-06-12 Mathias Lechelon , Marco Pettini

Diffusion models (DMs) have demonstrated remarkable success in real-world image super-resolution (SR), yet their reliance on time-consuming multi-step sampling largely hinders their practical applications. While recent efforts have…

Computer Vision and Pattern Recognition · Computer Science 2026-05-13 Jiaqi Xu , Wenbo Li , Haoze Sun , Fan Li , Zhixin Wang , Long Peng , Jingjing Ren , Haoran Yang , Xiaowei Hu , Renjing Pei , Pheng-Ann Heng