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We propose a 3D convolutional neural network to simultaneously segment and detect cell nuclei in confocal microscopy images. Mirroring the co-dependency of these tasks, our proposed model consists of two serial components: the first part…

Image and Video Processing · Electrical Eng. & Systems 2018-09-07 Sundaresh Ram , Vicky T. Nguyen , Kirsten H. Limesand , Mert R. Sabuncu

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

Image and Video Processing · Electrical Eng. & Systems 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

Image and Video Processing · Electrical Eng. & Systems 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Semantic segmentation for medical 3D image stacks enables accurate volumetric reconstructions, computer-aided diagnostics and follow up treatment planning. In this work, we present a novel variant of the Unet model called the NUMSnet that…

Image and Video Processing · Electrical Eng. & Systems 2023-04-07 Sohini Roychowdhury

Neuroimaging to neuropathology correlation (NTNC) promises to enable the transfer of microscopic signatures of pathology to in vivo imaging with MRI, ultimately enhancing clinical care. NTNC traditionally requires a volumetric MRI scan,…

Quantitative analysis of cell nuclei in microscopic images is an essential yet challenging source of biological and pathological information. The major challenge is accurate detection and segmentation of densely packed nuclei in images…

Quantitative Methods · Quantitative Biology 2019-11-14 Linqing Feng , Jun Ho Song , Jiwon Kim , Soomin Jeong , Jin Sung Park , Jinhyun Kim

Nuclei appear small in size, yet, in real clinical practice, the global spatial information and correlation of the color or brightness contrast between nuclei and background, have been considered a crucial component for accurate nuclei…

Image and Video Processing · Electrical Eng. & Systems 2023-07-18 Zhenqi He , Mathias Unberath , Jing Ke , Yiqing Shen

Convolutional Neural Networks (CNNs) have been recently employed to solve problems from both the computer vision and medical image analysis fields. Despite their popularity, most approaches are only able to process 2D images while most…

Computer Vision and Pattern Recognition · Computer Science 2016-06-16 Fausto Milletari , Nassir Navab , Seyed-Ahmad Ahmadi

Nuclei instance segmentation is an essential task in pathology image analysis, serving as the foundation for many downstream applications. The release of several public datasets has significantly advanced research in this area, yet many…

Image and Video Processing · Electrical Eng. & Systems 2025-08-22 Jiamu Wang , Jin Tae Kwak

Nucleus segmentation is a challenging task due to the crowded distribution and blurry boundaries of nuclei. Recent approaches represent nuclei by means of polygons to differentiate between touching and overlapping nuclei and have…

Computer Vision and Pattern Recognition · Computer Science 2023-03-17 Shengcong Chen , Changxing Ding , Minfeng Liu , Jun Cheng , Dacheng Tao

Abnormal iron accumulation in the brain subcortical nuclei has been reported to be correlated to various neurodegenerative diseases, which can be measured through the magnetic susceptibility from the quantitative susceptibility mapping…

Image and Video Processing · Electrical Eng. & Systems 2020-08-04 Chao Chai , Pengchong Qiao , Bin Zhao , Huiying Wang , Guohua Liu , Hong Wu , E Mark Haacke , Wen Shen , Chen Cao , Xinchen Ye , Zhiyang Liu , Shuang Xia

Robust and accurate nuclei centroid detection is important for the understanding of biological structures in fluorescence microscopy images. Existing automated nuclei localization methods face three main challenges: (1) Most of object…

Image and Video Processing · Electrical Eng. & Systems 2021-11-05 Liming Wu , Shuo Han , Alain Chen , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Identify the cells' nuclei is the important point for most medical analyses. To assist doctors finding the accurate cell' nuclei location automatically is highly demanded in the clinical practice. Recently, fully convolutional neural…

Computer Vision and Pattern Recognition · Computer Science 2019-11-25 Tianyang Zhang , Rui Ma

Whole brain segmentation is an important neuroimaging task that segments the whole brain volume into anatomically labeled regions-of-interest. Convolutional neural networks have demonstrated good performance in this task. Existing…

Image and Video Processing · Electrical Eng. & Systems 2021-11-01 Yeshu Li , Jonathan Cui , Yilun Sheng , Xiao Liang , Jingdong Wang , Eric I-Chao Chang , Yan Xu

Due to the wide existence and large morphological variances of nuclei, accurate nuclei instance segmentation is still one of the most challenging tasks in computational pathology. The annotating of nuclei instances, requiring experienced…

Computer Vision and Pattern Recognition · Computer Science 2020-07-23 Xinpeng Xie , Jiawei Chen , Yuexiang Li , Linlin Shen , Kai Ma , Yefeng Zheng

This study investigates a 3D and fully convolutional neural network (CNN) for subcortical brain structure segmentation in MRI. 3D CNN architectures have been generally avoided due to their computational and memory requirements during…

Computer Vision and Pattern Recognition · Computer Science 2017-04-21 J. Dolz , C. Desrosiers , I. Ben Ayed

Nuclei segmentation is a fundamental prerequisite in the digital pathology workflow. The development of automated methods for nuclei segmentation enables quantitative analysis of the wide existence and large variances in nuclei morphometry…

Computer Vision and Pattern Recognition · Computer Science 2024-01-17 Yi Lin , Zeyu Wang , Dong Zhang , Kwang-Ting Cheng , Hao Chen

Due to cellular heterogeneity, cell nuclei classification, segmentation, and detection from pathological images are challenging tasks. In the last few years, Deep Convolutional Neural Networks (DCNN) approaches have been shown…

Computer Vision and Pattern Recognition · Computer Science 2018-11-09 Md Zahangir Alom , Chris Yakopcic , Tarek M. Taha , Vijayan K. Asari

Microscopy imaging techniques are instrumental for characterization and analysis of biological structures. As these techniques typically render 3D visualization of cells by stacking 2D projections, issues such as out-of-plane excitation and…

Image and Video Processing · Electrical Eng. & Systems 2023-02-16 Amirkoushyar Ziabari , Derek C. Rose , Abbas Shirinifard , David Solecki

Accurate reconstruction of neuronal morphology is essential for classifying cell types and understanding brain connectivity. Recent advances in imaging and reconstruction techniques have greatly expanded the scale and quality of neuronal…

Neurons and Cognition · Quantitative Biology 2025-08-05 Wu Chen , Mingwei Liao , Xueyan Jia , Xiaowei Chen , Chi Xiao , Qingming Luo , Hui Gong , Anan Li
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