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Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites the fluorescence emission from a specific specimen position. Some actuators scan the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-10 Alessandro Zunino , Marco Castello , Giuseppe Vicidomini

Structured Illumination Microscopy (SIM) enables rapid, high-contrast optical sectioning of fresh tissue without staining or physical sectioning, making it promising for intraoperative and point-of-care diagnostics. Recent foundation and…

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…

Super-resolution microscopy in the visible is an established powerful tool in several disciplines. In the infrared (IR) spectral range, however, no comparable schemes have been demonstrated to date. In this work, we experimentally…

We present a study that evaluates the impact of regularization on the achieved resolution in restorations from a novel three-dimensional (3D) Structured Illumination Microscopy (3D-SIM) system with desirable tunability properties. This…

Image and Video Processing · Electrical Eng. & Systems 2024-03-22 Arash Atibi , Abdulaziz Alqahtani , Mohammed Younis , Chrysanthe Preza

Scanning Electron Microscopy (SEM) is pivotal in revealing intricate micro- and nanoscale features across various research fields. However, obtaining high-resolution SEM images presents challenges, including prolonged scanning durations and…

Image and Video Processing · Electrical Eng. & Systems 2024-10-08 Tom Reclik , Setareh Medghalchi , Philipp Schumacher , Maximilian Wollenweber , Talal Al-Samman , Sandra Korte-Kerzel , Ulrich Kerzel

In this work, we propose a structured illumination (SI) method based on a two-photon excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample…

Biological Physics · Physics 2016-09-16 Alex Bardales , Qinggele Li , Bingzhao Zhu , Xiaodong Tao , Marc Reinig , Joel Kubby

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase…

Optics · Physics 2025-09-01 James N. Caron

We demonstrate the combination of a hemispherical solid immersion lens with a micro-photoluminescence setup. Two advantages introduced by the SIL, an improved resolution of 0.4 times the wavelength in vacuum and a 5 times enhancement of the…

Optics · Physics 2009-11-07 S. Moehl , Hui Zhao , B. Dal Don , S. Wachter , H. Kalt

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To…

Image and Video Processing · Electrical Eng. & Systems 2021-06-07 Figen S. Oktem , Oğuzhan Fatih Kar , Can Deniz Bezek , Farzad Kamalabadi

It is now generally accepted that Euclidean-based metrics may not always adequately represent the subjective judgement of a human observer. As a result, many image processing methodologies have been recently extended to take advantage of…

Optimization and Control · Mathematics 2020-02-10 D. Otero , D. La Torre , O. Michailovich , E. R. Vrscay

The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing…

Optics · Physics 2022-02-18 S. Hossein S. Yaghoubi , Samira Ebrahimi , Masoomeh Dashtdar

We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the…

Ising machines are emerging as a powerful physical alternative to digital processors for solving combinatorial optimization problems. Among them, spatial photonic Ising machines (SPIMs) offer compact, room-temperature hardware with…

The recent increase in the extensive use of digital imaging technologies has brought with it a simultaneous demand for higher-resolution images. We develop a novel edge-informed approach to single image super-resolution (SISR). The SISR…

Image and Video Processing · Electrical Eng. & Systems 2019-09-13 Kamyar Nazeri , Harrish Thasarathan , Mehran Ebrahimi

Three-dimensional (3D) fluorescence imaging provides a vital approach for study of biological tissues with intricate structures, and optical sectioning structured illumination microscopy (OS-SIM) stands out for its high imaging speed, low…

A widely tested approach to overcoming the diffraction limit in microscopy without disturbing the sample relies on substituting widefield sample illumination with a structured light beam. This gives rise to confocal, image-scanning and…

Optics · Physics 2024-06-03 Alexander Duplinskiy , Jernej Frank , Kaden Bearne , A. I. Lvovsky

Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…

Optics · Physics 2024-12-19 Rui Ma , Olga Lyraki , Daniel Wehner , Jochen Guck

Image Super-Resolution (SR) is essential for a wide range of computer vision and image processing tasks. Investigating infrared (IR) image (or thermal images) super-resolution is a continuing concern within the development of deep learning.…

Image and Video Processing · Electrical Eng. & Systems 2025-09-25 Yongsong Huang , Tomo Miyazaki , Xiaofeng Liu , Shinichiro Omachi
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