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Quantitative susceptibility mapping (QSM) estimates the underlying tissue magnetic susceptibility from MRI gradient-echo phase signal and typically requires several processing steps. These steps involve phase unwrapping, brain volume…

Image and Video Processing · Electrical Eng. & Systems 2019-05-16 Hongjiang Wei , Steven Cao , Yuyao Zhang , Xiaojun Guan , Fuhua Yan , Kristen W. Yeom , Chunlei Liu

A universal methodology for coding-decoding the complex amplitude field of an imaged sample in coherent microscopy is presented, where no restrictions on any of the two interferometric beams are required. Thus, the imaging beam can be…

We present a technically simple implementation of quantitative phase imaging in confocal microscopy based on synthetic optical holography with sinusoidal-phase reference waves. Using a Mirau interference objective and low-amplitude vertical…

Quantitative Acoustic Microscopy (QAM) is an imaging technology utilising high frequency ultrasound to produce quantitative two-dimensional (2D) maps of acoustical and mechanical properties of biological tissue at microscopy scale.…

Image and Video Processing · Electrical Eng. & Systems 2024-09-23 Odysseas Pappas , Jonathan Mamou , Adrian Basarab , Denis Kouame , Alin Achim

Confocal Raman microscopy offers a particular pathway for monitoring chemical "fingerprints" of intracellular components like the cell membrane, organelles, and nucleus. Nevertheless, conventional Raman acquisitions of fixed or randomly…

Quantum phase is not a direct observable and is usually determined by interferometric methods. We present a method to map complete electron wave functions, including internal quantum phase information, from measured single-state probability…

Mesoscale and Nanoscale Physics · Physics 2008-03-19 Christopher R. Moon , Laila S. Mattos , Brian K. Foster , Gabriel Zeltzer , Wonhee Ko , Hari C. Manoharan

Non-contact scanning probe microscopy (SPM) has developed into a powerful technique to image many different properties of samples. The conventional method involves monitoring the amplitude, phase or frequency of a cantilever oscillating at…

Mesoscale and Nanoscale Physics · Physics 2015-06-16 Manan Mehta , Venkat Chandrasekhar

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

We present a simple, frequency domain, preprocessing step to Kirchhoff migration that allows the method to image scatterers when the wave field phase information is lost at the receivers, and only intensities are measured. The resulting…

Numerical Analysis · Mathematics 2016-09-21 Patrick Bardsley , Fernando Guevara Vasquez

Quantitative phase imaging (QPI) is a valuable label-free modality that has gained significant interest due to its wide potentials, from basic biology to clinical applications. Most existing QPI systems measure microscopic objects via…

Optics · Physics 2021-12-22 Chenfei Hu , Mikhail E. Kandel , Young Jae Lee , Gabriel Popescu

For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent…

Optics · Physics 2017-05-31 Chao Zuo , Jiasong Sun , Jiaji Li , Jialin Zhang , Anand Asundi , Qian Chen

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

Quantitative Methods · Quantitative Biology 2025-11-03 Surajit Das , Pavel Zun

This paper presents a microfluidic device that implements standing surface acoustic waves in order to handle single cells, droplets, and generally particles. The particles are moved in a very controlled manner by the two-dimensional…

Fluid Dynamics · Physics 2012-11-14 S. B. Q. Tran , Philippe Marmottant , Pierre Thibault

A simple implementation of Kelvin probe force microscopy is reported that enables recording topographic images in the absence of any component of the electrostatic force. Our approach is based on a close loop z-spectroscopy operated in data…

Materials Science · Physics 2023-03-22 Aloïs Arrighi , Nathan Ullberg , Vincent Derycke , Benjamin Grévin

We report Tensorial Tomographic Differential Phase-Contrast microscopy (T2DPC), a quantitative label-free tomographic imaging method for simultaneous measurement of phase and anisotropy. T2DPC extends differential phase-contrast microscopy,…

The flexible endoscope is a minimally invasive tool in clinical settings, but most of them rely on exogenous staining for diagnosis to provide qualitative information. Here, we demonstrated a flexible endoscopic microscopy (FEM) with…

Quantitative phase imaging (QPI) recovers the exact wavefront of light from the intensity measured by a camera. Topographical maps of translucent microscopic bodies can be extracted from these quantified phase shifts. We demonstrate…

Intermodulation atomic force microscopy (IMAFM) is a dynamic mode of atomic force microscopy (AFM) with two-tone excitation. The oscillating AFM cantilever in close proximity to a surface experiences the nonlinear tip-sample force which…

Mesoscale and Nanoscale Physics · Physics 2013-02-08 Daniel Platz , Erik A. Tholen , Carsten Hutter , Arndt C. von Bieren , David B. Haviland

Quadrature amplitude modulation (QAM) and a complementary representation of a causal waveform have been used to develop a sidelobe-free pulse-compression technique. Envelopes of radio-frequency (RF) pulses under study include both unimodal…

Signal Processing · Electrical Eng. & Systems 2021-10-20 W. J. Szajnowski

Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…

Optics · Physics 2020-11-11 Xianye Li , Yafei sun , Yikang He , Xun Li , Baoqing Sun