Tensorial tomographic differential phase-contrast microscopy
Abstract
We report Tensorial Tomographic Differential Phase-Contrast microscopy (T2DPC), a quantitative label-free tomographic imaging method for simultaneous measurement of phase and anisotropy. T2DPC extends differential phase-contrast microscopy, a quantitative phase imaging technique, to highlight the vectorial nature of light. The method solves for permittivity tensor of anisotropic samples from intensity measurements acquired with a standard microscope equipped with an LED matrix, a circular polarizer, and a polarization-sensitive camera. We demonstrate accurate volumetric reconstructions of refractive index, birefringence, and orientation for various validation samples, and show that the reconstructed polarization structures of a biological specimen are predictive of pathology.
Cite
@article{arxiv.2204.11397,
title = {Tensorial tomographic differential phase-contrast microscopy},
author = {Shiqi Xu and Xiang Dai and Xi Yang and Kevin C. Zhou and Kanghyun Kim and Vinayak Pathak and Carolyn Glass and Roarke Horstmeyer},
journal= {arXiv preprint arXiv:2204.11397},
year = {2022}
}