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Phase imaging is gaining importance due to its applications in fields like biomedical imaging and material characterization. In biomedical applications, it can provide quantitative information missing in label-free microscopy modalities.…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Gabriel della Maggiora , Luis Alberto Croquevielle , Harry Horsley , Thomas Heinis , Artur Yakimovich

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Quantitative phase imaging (QPI) enables visualization and quantitative extraction of the optical phase information of transparent samples. However, conventional QPI techniques typically rely on multi-frame acquisition or complex…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Resistive Pulse Sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells,…

Biological Physics · Physics 2020-04-01 Antoine Riaud , Anh L. P. Thai , Wei Wang , Valerie Taly

Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques and are widely used for biomedical applications. Each method, however, possesses distinct limitations: QPM,…

Optics · Physics 2025-03-20 Kohki Horie , Keiichiro Toda , Takuma Nakamura , Takuro Ideguchi

Quantitative phase imaging (QPI) is a label-free computational imaging technique used in various fields, including biology and medical research. Modern QPI systems typically rely on digital processing using iterative algorithms for phase…

Optics · Physics 2023-06-28 Yuhang Li , Yi Luo , Deniz Mengu , Bijie Bai , Aydogan Ozcan

Optical tomographic imaging of biological specimen bases its reliability on the combination of both accurate experimental measures and advanced computational techniques. In general, due to high scattering and absorption in most of the…

We extend the recently introduced phaseless auxiliary-field quantum Monte Carlo (QMC) approach to any single-particle basis, and apply it to molecular systems with Gaussian basis sets. QMC methods in general scale favorably with system…

Computational Physics · Physics 2007-05-23 W. A. Al-Saidi , Shiwei Zhang , Henry Krakauer

Quantitative imaging of subcellular processes in living embryos, stem-cell systems, and organoid models requires microscopy platforms that combine high spatial resolution, fast volumetric acquisition, long-term stability, and minimal…

Quantitative Methods · Quantitative Biology 2025-12-30 Armin Shoushtarizadeh , Michele Cerminara , Corinne Chureau , Leah Friedman , Deepthi Kailash , Thomas Gregor

We demonstrate a single-arm optical platform for phase-retrieval-free, quantitative dynamic phase mapping of continuous transparent media via field-correlation ghost imaging. By modeling the medium as a dynamic pure-phase object, we…

Optics · Physics 2026-03-24 Chaoran Wang , Jinquan Qi , Shuang Liu , Xingzhao Jiang , Shensheng Han

This paper reported a general noninterferometric high-accuracy quantitative phase imaging (QPI) method for arbitrary complex valued objects. Given by a typical 4f optical configuration as the imaging system, three frames of small-window…

Image and Video Processing · Electrical Eng. & Systems 2021-05-05 Jianhui Huang , An Pan , Huiliang Jin , Guoxiang Meng , Qian Ye

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…

Fluid Dynamics · Physics 2017-09-19 S. Quint , A. F. Christ , A. Guckenberger , S. Himbert , L. Kaestner , S. Gekle , C. Wagner

Imaging of biological cells and tissues often relies on fluorescent labels, which offer high contrast with molecular specificity. The use of exogenous labeling agents, however, may alter the normal physiology of the bio-specimens.…

Instrumentation and Detectors · Physics 2019-10-01 Zichao Bian , Shaowei Jiang , Pengming Song , He Zhang , Pouria Hoveida , Kazunori Hoshino , Guoan Zheng

The constant interplay and information exchange between cells and their micro-environment are essential to their survival and ability to execute biological functions. To date, a few leading technologies such as traction force microscopy,…

Cell Behavior · Quantitative Biology 2023-06-29 Feng Xu , Shuxiang Zhang , Linjie Ma , Yong Hou , Jie Li , Andrej Denisenko , Zifu Li , Joachim Spatz , Jörg Wrachtrup , Qiang Wei , Zhiqin Chu

This paper describes continuously phase-modulated standing surface acoustic waves (CPM-SSAW) and its application for particle separation in multiple pressure nodes. A linear change of phase in CPM-SSAW applies a force to particles whose…

Fluid Dynamics · Physics 2017-04-05 Junseok Lee , Chanryeol Rhyou , Byungjun Kang , Hyungsuk Lee

Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source…

We report the details of construction and testing of a Quantum Twisting Microscope, a recently developed scanning probe instrument that enables twist angle dependent electronic measurements on layered materials. Our implementation is based…

Mesoscale and Nanoscale Physics · Physics 2026-04-07 Sayanwita Biswas , Ranjani Ramachandran , Patrick Irvin , Jeremy Levy

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

We present a data-driven approach to compensate for optical aberration in calibration-free quantitative phase imaging (QPI). Unlike existing methods that require additional measurements or a background region to correct aberrations, we…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Taean Chang , Youngju Jo , Gunho Choi , Donghun Ryu , Hyun-Seok Min , Yongkeun Park