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Related papers: Spatial light interference microscopy (SLIM): prin…

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Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Phase imaging techniques extract the optical path-length information of a scene, whereas wavefront sensors provide the shape of an optical wavefront. Since these two applications have different technical requirements, they have developed…

Optics · Physics 2018-02-28 F. Soldevila , V. Durán , P. Clemente , J. Lancis , E. Tajahuerce

We introduce the white light quantitative phase imaging unit (WQPIU) as a practical realization of quantitative phase imaging (QPI) on standard microscope platforms. The WQPIU is a compact stand-alone unit which measures sample induced…

Optics · Physics 2016-05-04 YoonSeok Baek , KyeoReh Lee , Jonghee Yoon , Kyoohyun Kim , YongKeun Park

Quantitative phase microscopy (QPM) enables label-free measurement of local optical path length variations, providing critical insight into the structure and dynamics of transparent biological specimens. Here, a highly sensitive lateral…

We demonstrate two-step phase-shifting interferometry (holography) of complex laser modes generated by a spatial light modulator (SLM), in which the amplitude and phase of the signal are determined directly from measurements of…

Optics · Physics 2024-09-16 Lark E. Bradsby , Andrew A. Voitiv , Mark E. Siemens

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

Computer Vision and Pattern Recognition · Computer Science 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

This paper presents for the first time an innovative instrument called an inverted scanning microwave microscope (iSMM), which is capable of noninvasive and label-free imaging and characterization of intracellular structures of a live cell…

Photoluminesce (PL) spectroscopy offers excellent methods for mapping the PL decay on the nanosecond time scale. However, capturing maps of emission dynamics on the microsecond time scale can be highly time-consuming. We present a new…

Optics · Physics 2021-02-24 Jiri Junek , Karel Zidek

Label-free imaging approaches seek to simplify and augment histopathologic assessment by replacing the current practice of staining by dyes to visualize tissue morphology with quantitative optical measurements. Quantitative phase imaging…

Quantitative phase microscopy (QPM) is a label-free technique that enables to monitor morphological changes at subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence…

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…

High-performance programmable silicon photonic circuits are considered to be a critical part of next generation architectures for optical processing, photonic quantum circuits and neural networks. Low-loss optical phase change materials…

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

Image and Video Processing · Electrical Eng. & Systems 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

We present a simple and effective method to eliminate system aberrations and speckle noise in quantitative phase imaging. Using spiral integration, complete information about system aberration is calculated from three laterally shifted…

Biological Physics · Physics 2018-02-14 Inhyeok Choi , Kyeoreh Lee , YongKeun Park

Phase contrast microscopy (PCM) has been widely used in biomedicine research, which allows users to observe objectives without staining or killing them. One important related research is to employ PCM to monitor live cells. How to segment…

Computer Vision and Pattern Recognition · Computer Science 2019-11-26 Lin Zhang

Photonic integrated circuits (PICs) play a pivotal role in many applications. Particularly powerful are circuits based on meshes of reconfigurable Mach-Zehnder interferometers as they enable active processing of light. Various possibilities…

We propose a new benchmark for evaluating stereoscopic visual-inertial computer vision algorithms (SLAM/ SfM/ 3D Reconstruction/ Visual-Inertial Odometry) for minimally invasive surgical (MIS) interventions in the abdomen. Our MITI Dataset…

Image and Video Processing · Electrical Eng. & Systems 2026-02-12 Regine Hartwig , Daniel Ostler , Jean-Claude Rosenthal , Hubertus Feußner , Dirk Wilhelm , Dirk Wollherr

Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane…

Quantitative phase imaging (QPI) is a label-free computational imaging technique used in various fields, including biology and medical research. Modern QPI systems typically rely on digital processing using iterative algorithms for phase…

Optics · Physics 2023-06-28 Yuhang Li , Yi Luo , Deniz Mengu , Bijie Bai , Aydogan Ozcan

Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source…