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Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…

Image and Video Processing · Electrical Eng. & Systems 2020-03-26 Charles N. Christensen , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Cross-grating phase microscopy (CGM) is a quantitative phase microscopy technique based on the association of a 2-dimensional diffraction grating (cross-grating) and a regular camera sensor, separated by a millimetric distance. This simple…

Optics · Physics 2024-11-04 Baptiste Marthy , Guillaume Baffou

The development of advanced packaging is essential in the semiconductor manufacturing industry. However, non-destructive testing (NDT) of advanced packaging becomes increasingly challenging due to the depth and complexity of the layers…

Instrumentation and Detectors · Physics 2025-07-17 J. Senthilnath , Jayasanker Jayabalan , Zhuoyi Lin , Aye Phyu Phyu Aung , Chen Hao , Kaixin Xu , Yeow Kheng Lim , F. C. Wellstood

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

This paper presents a simple method to recover phase information from regions of unresolvable fringe density, specifically, at the camera corners, where interference fringes are not resolved by the camera using Phase Shifting Interferometry…

Optics · Physics 2026-03-17 Indrani Bhattacharya

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

Data Analysis, Statistics and Probability · Physics 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Scanning superconducting quantum interference device microscopy (SSM) is a scanning probe technique that images local magnetic flux, which allows for mapping of magnetic fields with high field and spatial accuracy. Many studies involving…

Instrumentation and Detectors · Physics 2018-01-17 P. Reith , X. Renshaw Wang , H. Hilgenkamp

We present cytometric classification of live healthy and cancer cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor…

Quantitative Methods · Quantitative Biology 2020-01-23 Darina Roitshtain , Lauren Wolbromsky , Evgeny Bal , Hayit Greenspan , Lisa L. Satterwhite , Natan T. Shaked

Modern optical microscopy methods have been advanced to provide super resolution at high imaging speed, but not chirality discriminative. We recently proposed chiral structured-illumination microscopy (SIM) method to image chiral…

Optics · Physics 2021-03-11 Jiwei Zhang , Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…

Optics · Physics 2020-01-08 Samira Ebrahimi , Masoomeh Dashtdar

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

This book chapter reviews progress in crystallographic image processing (CIP) for scanning probe microscopy (SPM) that has occurred since our description of the technique was first put into open access in this book series in the year 2010.…

Materials Science · Physics 2020-12-02 Peter Moeck

In Super-resolution, a varying-illumination image stack is required. This enriched the dataset typically necessitates precise mechanical control and micron scale optical alignment and repeatability. Here, we introduce a novel methodology…

Laser Interference Lithography (LIL) is a versatile fabrication method for patterning sub-micron structures in arrays covering large areas. It is a facile and fast mask-less lithography process to produce large area periodic patterns. The…

Optics · Physics 2014-08-28 A. Alfred Kiruba Raj , D. Jackuline Moni , D. Devaprakasam

Deficient myelination of the brain is associated with neurodevelopmental delays, particularly in high-risk infants, such as those born small in relation to their gestational age (SGA). New methods are needed to further study this condition.…

Integrated photonics has emerged as one of the most promising platforms for quantum applications. The performances of quantum photonic integrated circuits (QPIC) necessitate a demanding optimization to achieve enhanced properties and…

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

Data Analysis, Statistics and Probability · Physics 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

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