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Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

Coherent diffractive imaging is unique as the only route for achieving diffraction-limited spatial resolution in the extreme ultraviolet and X-ray regions, limited only by the wavelength of the light. Recently, advances in coherent short…

Far-field characterization of small objects is severely constrained by the diffraction limit. Existing tools achieving sub-diffraction resolution often utilize point-by-point image reconstruction via scanning or labelling. Here, we present…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies…

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Parallel imaging is ubiquitous in MRI, enabling diverse applications such as ultra-high-resolution functional and quantitative imaging with greater temporal resolution or reduced scan times respectively. Successful unfolding is contingent…

Scanning Electron Microscopy (SEM) is pivotal in revealing intricate micro- and nanoscale features across various research fields. However, obtaining high-resolution SEM images presents challenges, including prolonged scanning durations and…

Image and Video Processing · Electrical Eng. & Systems 2024-10-08 Tom Reclik , Setareh Medghalchi , Philipp Schumacher , Maximilian Wollenweber , Talal Al-Samman , Sandra Korte-Kerzel , Ulrich Kerzel

High-spatial-resolution vibrational spectroscopy is one of the principal techniques for nanoscale compositional analysis in biological materials. Here, we present a new method for the analysis of whole-cell biological specimens through…

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

In this article, we provide an alternative up-sampling and PSF deconvolution method for the iterative multi-exposure coaddition. Different from the previous works, the new method has a ratio-correction term, which allows the iterations to…

Instrumentation and Methods for Astrophysics · Physics 2022-10-26 Lei Wang , Guoliang Li , Xi Kang

We develop an imaging algorithm that exploits strong scattering to achieve super-resolution in changing random media. The method processes large and diverse array datasets using sparse dictionary learning, clustering, and multidimensional…

Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

While burst Low-Resolution (LR) images are useful for improving their Super Resolution (SR) image compared to a single LR image, prior burst SR methods are trained in a deterministic manner, which produces a blurry SR image. Since such…

Computer Vision and Pattern Recognition · Computer Science 2025-07-21 Kento Kawai , Takeru Oba , Kyotaro Tokoro , Kazutoshi Akita , Norimichi Ukita

Characterization of microstructures in live tissues is one of the keys to diagnosing early stages of pathology and understanding disease mechanisms. However, the extraction of reliable information on biomarkers based on microstructure…

Quantum Physics · Physics 2020-09-01 Analia Zwick , Dieter Suter , Gershon Kurizki , Gonzalo A. Alvarez

Advances in intensity-based microscopy techniques have improved our ability to quantify particle motion at microscopic scales, enabling insight into diffusion and collective dynamics. Building on this foundation, we introduce a novel…

Soft Condensed Matter · Physics 2026-04-03 Sophie Hermann , Seyed Saman Banarooei , Adam Carter , Carlos A. Silvera Batista , Sophie Marbach