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The photo-kinetics of fluorescent molecules have enabled the circumvention of far-field optical diffraction-limit. Despite its enormous potential, the necessity to label the sample may adversely influence the delicate biology under…

Volume electron microscopy is the method of choice for the in-situ interrogation of cellular ultrastructure at the nanometer scale. Recent technical advances have led to a rapid increase in large raw image datasets that require…

Computer Vision and Pattern Recognition · Computer Science 2023-03-08 Andreas Müller , Deborah Schmidt , Lucas Rieckert , Michele Solimena , Martin Weigert

High-index dielectric nanoparticles have become a powerful platform for modern light science, enabling various fascinating applications, especially in nonlinear nanophotonics for which they enable special types of optical nonlinearity, such…

The polarization of the light scattered by an optically dense, random solution of dielectric nanoparticles shows peculiar properties when the scatterers exhibit strong electric and magnetic polarizabilities. While the distribution of the…

Optical levitation of dielectric particles in vacuum is a powerful technique for precision measurements, testing fundamental physics, and quantum information science. Conventional optical tweezers require bulky optical components for…

Imaging of the optical properties of individual nanosystems beyond fluorescence can provide a wealth of information. However, the minute signals for absorption and dispersion are challenging to observe, and only specialized techniques…

Optics · Physics 2015-09-10 Matthias Mader , Jakob Reichel , Theodor W. Hänsch , David Hunger

Fast detection and characterization of single nanoparticles such as viruses, airborne aerosols and colloidal particles are considered to be particularly important for medical applications, material science and atmospheric physics. In…

The ideal imaging system would efficiently capture information about all fundamental properties light: intensity, direction, wavelength, and polarization. Most common imaging systems only map the spatial degrees of freedom of light onto a…

Optics · Physics 2023-01-26 Conner Ballew , Gregory Roberts , Andrei Faraon

We propose an autofocusing algorithm to obtain, relatively accurately, the 3D position of each particle, particularly its axial location, and particle number of a dense transparent particle solution via its hologram. First, morphological…

Signal Processing · Electrical Eng. & Systems 2025-03-25 Wei-Na Li , Yi Zhou , Jiatai Chen , Hongjie Ou , XiangSheng Xie

The holographic mask technique is used to create freely moving electrons with quantized angular momentum. With electron optical elements they can be focused to vortices with diameters below the nanometer range. The understanding of these…

Materials Science · Physics 2013-04-09 P. Schattschneider , M. Stöger-Pollach , S. Löffler , A. Steiger-Thirsfeld , J. Hell , J. Verbeeck

Absorption microscopy is a powerful technique, enabling the detection of single non- fluorescent molecules at room temperature. So far, the molecular absorption has been probed optically via the attenuation of a probing laser. The…

Applied Physics · Physics 2018-11-21 Miao-Hsuan Chien , Mario Brameshuber , Gerhard J. Schütz , Silvan Schmid

Second harmonic generation (SHG) is a nonlinear optical response arising exclusively from broken inversion symmetry in the electric-dipole limit. Recently, SHG has attracted widespread interest as a versatile and noninvasive tool for…

Label-free observation of nanoparticles by far-field optical microscopy is challenging because their ability to scatter or absorb light dramatically diminishes with decreasing size. Surface plasmon resonance (SPR) and localized surface…

Optics · Physics 2020-12-02 Nareg Ohannesian , Ibrahim Misbah , Steven H Lin , Wei-Chuan Shih

We propose a scheme for detecting and differentiating deeply subwavelength particles based on their spatial features. Our approach combines scattering from an ultrasonically modulated nanopatterend grating with heterodyne techniques to…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

High dynamic range (HDR) images are important for a range of tasks, from navigation to consumer photography. Accordingly, a host of specialized HDR sensors have been developed, the most successful of which are based on capturing variable…

Image and Video Processing · Electrical Eng. & Systems 2023-08-17 Mingyang Xie , Matthew Chan , Christopher Metzler

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40…

Accurately measuring cell stiffness is challenging due to the invasiveness of traditional methods like atomic force microscopy (AFM) and optical stretching. We introduce a non-invasive off-axis system using holographic imaging and acoustic…

Currently the extent of nanoplastic in the environment can only be estimated by extrapolation from the plastic waste that can be detected. To be able to quantify the whole extent of the problem, detection methods have to be developed that…

Optics · Physics 2025-11-12 Ambika Shorny , Fritz Steiner , Helmut Hörner , Sarah M. Skoff

Live cell imaging using metallic nanoparticles as tags is an emerging technique to visualize long and highly dynamic processes due to the lack of photobleaching and high photon rate. However, the lack of excited states as compared to…

Mesoscale and Nanoscale Physics · Physics 2011-01-12 H. E. Grecco , O. E. Martínez

Atomic-resolution imaging with scanning transmission electron microscopy is a powerful tool for characterizing the nanoscale structure of materials, in particular features such as defects, local strains, and symmetry-breaking distortions.…