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Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…
Two-photon microscopy (TPM) enables deep tissue imaging but requires excitation pulses that have a large product of average and peak power, typically supplied by femtosecond solid-state lasers. However, these lasers are bulky and…
Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…
An exciting recent development for deep-tissue imaging with cellular resolution is three-photon fluorescence microscopy (3PM) with excitation at long wavelengths (1300 and 1700 nm). In the last few years, long-wavelength 3PM has driven…
We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…
Multiphoton microscopy has enabled us to image cellular dynamics in vivo. However, the excitation wavelength for imaging with commercially available lasers is mostly limited between 650-1040 nm. Here we develop a femtosecond fiber laser…
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…
Many experiments in biological and medical sciences currently use multiphoton microscopy as a core imaging technique. To date, solid-state lasers are most commonly used as excitation beam sources. However, the most demanding applications…
In this paper, we introduce Holographic Spectral Multiplexing (HSM) as a novel technique to enable multiple-input multiple-output (MIMO) communication in optical networks. HSM uses the spectral space of ultrashort laser pulses to create…
Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…
Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz),…
Full-color imaging is significant in digital pathology. Compared with a grayscale image or a pseudo-color image that only contains the contrast information, it can identify and detect the target object better with color texture information.…
Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source…
Pathological features are the gold standard for tumor diagnosis, guiding treatment and prognosis. However, standard histopathological process is labor-intensive and time-consuming, while frozen sections have lower accuracy. Dynamic…
Fourier ptychographic microscopy (FPM) is a pivotal computational imaging technique that achieves phase and amplitude reconstruction with high resolution and wide field of view, using low numerical aperture objectives and LED array…
Multiplex imaging is revolutionizing pathology by enabling the simultaneous visualization of multiple biomarkers within tissue samples, providing molecular-level insights that traditional hematoxylin and eosin (H&E) staining cannot provide.…
Today the gold standard for in vivo imaging through scattering tissue is the point-scanning two-photon microscope (PSTPM). Especially in neuroscience, PSTPM is widely used for deep-tissue imaging in the brain. However, due to sequential…
Three-dimensional (3D) histopathology of unprocessed tissues has the potential to transform disease management by enabling volumetric characterization of tissue microarchitecture and in-vivo assessment. Back-illumination Interference…
Immunohistochemical (IHC) staining provides crucial molecular characterization of tissue samples and plays an indispensable role in the clinical examination and diagnosis of cancers. However, compared with the commonly used Hematoxylin and…
Fourier ptychographic microscopy (FPM) is a computational approach geared towards creating high-resolution and large field-of-view images without mechanical scanning. To acquire color images of histology slides, it often requires sequential…