Related papers: Virtual H&E Histology by Fiber-Based Picosecond Tw…
Histological examination is crucial for cancer diagnosis, including hematoxylin and eosin (H&E) staining for mapping morphology and immunohistochemistry (IHC) staining for revealing chemical information. Recently developed two-color…
Detection and identification of lipids are highly coveted for the interrogation of chronic diseases such as atherosclerosis and myocardial infarction. Intravascular photoacoustic imaging (IVPA) and deep tissue imaging are modern techniques,…
Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation…
Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…
A femtosecond frequency-doubled Erbium-doped fiber laser with adjustable pulse repetition rate is developed and applied in two-photon excited fluorescence microscopy. The all-fiber laser system provides the fundamental pulse at 1560 nm…
Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…
The escalating data volume and complexity resulting from the rapid expansion of artificial intelligence (AI), internet of things (IoT) and 5G/6G mobile networks is creating an urgent need for energy-efficient, scalable computing hardware.…
Structured Illumination Microscopy (SIM) enables rapid, high-contrast optical sectioning of fresh tissue without staining or physical sectioning, making it promising for intraoperative and point-of-care diagnostics. Recent foundation and…
We present HSIM: a dedicated pipeline for simulating observations with the HARMONI integral field spectrograph on the European Extremely Large Telescope. HSIM takes high spectral and spatial resolution input data-cubes, encoding physical…
Smoothing filter is the method of choice for image preprocessing and pattern recognition. We present a new concurrent method for smoothing 2D object in binary case. Proposed method provides a parallel computation while preserving the…
We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…
Confocal laser endomicroscopy (CLE) allow on-the-fly in vivo intraoperative imaging in a discreet field of view, especially for brain tumors, rather than extracting tissue for examination ex vivo with conventional light microscopy.…
An established technique for the measurement of ultra-high-energy-cosmic-rays is the detection of the fluorescence light induced in the atmosphere of the Earth, by means of telescopes equipped with photomultiplier tubes. Silicon…
Two-photon absorption (TPA) fluorescence of biomarkers has been decisive in advancing the fields of biosensing and deep-tissue in vivo imaging of live specimens. However, due to the extremely small TPA cross section and the quadratic…
In this work we demonstrate 3D imaging using two-photon excitation through a 20 cm long multimode optical fiber (MMF) of 350 micrometers diameter. The imaging principle is similar to single photon fluorescence through a MMF, except that a…
HWO aims to directly image objects orbiting Sun-like stars, using a 6-m telescope capable of high-contrast imaging ($10^{-10}$) and spectroscopy to search for biosignatures in planets located in the habitable zone. Recent laboratory…
We demonstrate a new scheme of spectromicroscopy in the extreme ultraviolet (EUV) spectral range, where the spectral response of the sample at different wavelengths is imaged simultaneously. It is enabled by applying ptychographical…
Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…
Thin-film lithium niobate (TFLN) is a widely used platform for nonlinear frequency conversion, as its strong nonlinear susceptibility and enhanced modal confinement intensify nonlinear interactions. Frequency doubling from NIR to visible…
The performance of the $200\times2.5\times1$ cm$^3$ plastic scintillator strip with wavelength shifting fiber read-out by two novel photodetectors called Silicon PhotoMultipliers (SiPMs) is discussed. The advantages of SiPM relative to the…