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The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. This manuscript reports the development of Polarized Localization Microscopy (PLM),…

Biological Physics · Physics 2017-03-31 Abir Maarouf Kabbani , Christopher V. Kelly

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

We present a method for speckle holography that is optimised for crowded fields. Its two key features are an iterativ improvement of the instantaneous Point Spread Functions (PSFs) extracted from each speckle frame and the (optional)…

Instrumentation and Methods for Astrophysics · Physics 2015-05-30 R. Schoedel , S. Yelda , A. Ghez , J. H. V. Girard , L. Labadie , R. Rebolo , A. Perez-Garrido , M. R. Morris

In continuous-variable tomography, with finite data and limited computation resources, reconstruction of a quantum state of light is performed on a finite-dimensional subspace. No systematic method was ever developed to assign such a…

The proposed next-generation Very Large Array (ngVLA) will enable the imaging of astronomical sources in unprecedented detail by providing an order of magnitude improvement in sensitivity and angular resolution compared with radio…

Solar and Stellar Astrophysics · Physics 2019-10-23 Kazunori Akiyama , Lynn D. Matthews

Unsupervised clustering algorithm can effectively reduce the dimension of high-dimensional unlabeled data, thus reducing the time and space complexity of data processing. However, the traditional clustering algorithm needs to set the upper…

Machine Learning · Computer Science 2022-01-17 Zecang Gu , Xiaoqi Sun , Yuan Sun , Fuquan Zhang

Single-beam scanning microscopy (SBSM) is one of the most robust strategies for commercial optical systems. Although structured illumination combined with Fourier-domain spatial spectrum fusion can enhance SBSM resolution beyond the…

Optics · Physics 2025-07-15 Yongtao Liu , Tianxiao Wu , Xiao Zhou , Fan Wang

Single-image super-resolution (SISR) is a canonical problem with diverse applications. Leading methods like SRGAN produce images that contain various artifacts, such as high-frequency noise, hallucinated colours and shape distortions, which…

Machine Learning · Computer Science 2018-10-03 Ke Li , Shichong Peng , Jitendra Malik

We formulate and investigate a statistical inverse problem of a random tomographic nature, where a probability density function on $\mathbb{R}^3$ is to be recovered from observation of finitely many of its two-dimensional projections in…

Statistics Theory · Mathematics 2009-09-03 Victor M. Panaretos

The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…

Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

Point-spread function (PSF) estimation in spatially undersampled images is challenging because large pixels average fine-scale spatial information. This is problematic when fine-resolution details are necessary, as in optimal photometry…

Instrumentation and Methods for Astrophysics · Physics 2021-02-03 Teresa Symons , Michael Zemcov , James Bock , Yun-Ting Cheng , Brendan Crill , Christopher Hirata , Stephanie Venuto

Precise photometric and astrometric measurements on astronomical images require an accurate knowledge of the Point Spread Function (PSF). When the PSF cannot be modelled directly from the image, PSF-reconstruction techniques become the only…

Instrumentation and Methods for Astrophysics · Physics 2020-02-05 D. Massari , A. Marasco , O. Beltramo-Martin , J. Milli , G. Fiorentino , E. Tolstoy , F. Kerber

Single image super resolution (SR) has seen major performance leaps in recent years. However, existing methods do not allow exploring the infinitely many plausible reconstructions that might have given rise to the observed low-resolution…

Computer Vision and Pattern Recognition · Computer Science 2020-06-23 Yuval Bahat , Tomer Michaeli

We propose a method for the unsupervised clustering of hyperspectral images based on spatially regularized spectral clustering with ultrametric path distances. The proposed method efficiently combines data density and geometry to…

Computer Vision and Pattern Recognition · Computer Science 2020-04-13 Shukun Zhang , James M. Murphy

One of the challenges in single particle reconstruction in cryo-electron microscopy is to find a three-dimensional model of a molecule using its two-dimensional noisy projection-images. In this paper, we propose a robust "angular…

Computational Geometry · Computer Science 2020-01-08 Gabi Pragier , Yoel Shkolnisky

In single-molecule microscopy it is necessary to locate with high precision point sources from noisy observations of the spectrum of the signal at frequencies capped by $f_c$, which is just about the frequency of natural light. This paper…

Information Theory · Computer Science 2015-04-06 Veniamin I. Morgenshtern , Emmanuel J. Candes

Supervoxel methods such as Simple Linear Iterative Clustering (SLIC) are an effective technique for partitioning an image or volume into locally similar regions, and are a common building block for the development of detection, segmentation…

Computer Vision and Pattern Recognition · Computer Science 2017-02-10 Benjamin Irving

Scanning tunnelling microscopy (STM) enables atomic-resolution imaging and atom manipulation, but its utility is often limited by tip degradation and slow serial data acquisition. Fabrication adds another layer of complexity since the tip…

Computer Vision and Pattern Recognition · Computer Science 2025-10-31 Nikola L. Kolev , Tommaso Rodani , Neil J. Curson , Taylor J. Z. Stock , Alberto Cazzaniga