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The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel

We propose an unfolded accelerated projected-gradient descent procedure to estimate model and algorithmic parameters for image super-resolution and molecule localization problems in image microscopy. The variational lower-level constraint…

Numerical Analysis · Mathematics 2024-03-27 Silvia Bonettini , Luca Calatroni , Danilo Pezzi , Marco Prato

Single-image super-resolution (SISR) typically focuses on restoring various degraded low-resolution (LR) images to a single high-resolution (HR) image. However, during SISR tasks, it is often challenging for models to simultaneously…

Image and Video Processing · Electrical Eng. & Systems 2023-11-10 Xin Wang , Jing-Ke Yan , Jing-Ye Cai , Jian-Hua Deng , Qin Qin , Yao Cheng

Maximum-likelihood methods are applied to the problem of absorption tomography. The reconstruction is done with the help of an iterative algorithm. We show how the statistics of the illuminating beam can be incorporated into the…

Data Analysis, Statistics and Probability · Physics 2009-11-07 J. Rehacek , Z. Hradil , M. Zawisky , W. Treimer , M. Strobl

The Constrained Minimal Supersymmetric Standard Model (CMSSM) is one of the simplest and most widely-studied supersymmetric extensions to the standard model of particle physics. Nevertheless, current data do not sufficiently constrain the…

High Energy Physics - Phenomenology · Physics 2015-03-13 Yashar Akrami , Pat Scott , Joakim Edsjö , Jan Conrad , Lars Bergström

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…

Optics · Physics 2018-05-03 Elias Nehme , Lucien E. Weiss , Tomer Michaeli , Yoav Shechtman

Image superresolution involves the processing of an image sequence to generate a still image with higher resolution. Classical approaches, such as bayesian MAP methods, require iterative minimization procedures, with high computational…

Computer Vision and Pattern Recognition · Computer Science 2016-08-31 Carlos Miravet , Francisco B. Rodriguez

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

Super-resolution microscopy is providing unprecedented insights into biology by resolving details much below the diffraction limit. State-of-the-art Single Molecule Localization Microscopy (SMLM) techniques for super-resolution are…

Quantitative Methods · Quantitative Biology 2016-12-13 Krishna Agarwal , Radek Macháň

Diffusion models are the current state-of-the-art for solving inverse problems in imaging. Their impressive generative capability allows them to approximate sampling from a prior distribution, which alongside a known likelihood function…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Samuel W. Remedios , Zhangxing Bian , Shuwen Wei , Aaron Carass , Jerry L. Prince , Blake E. Dewey

We propose a continuous non-convex variational model for Single Molecule Localisation Microscopy (SMLM) super-resolution in order to overcome light diffraction barriers. Namely, we consider a variation of the Continuous Exact $\ell_0$…

Image and Video Processing · Electrical Eng. & Systems 2020-10-27 Marta Lazzaretti , Luca Calatroni , Claudio Estatico

The ensemble average of physical properties of molecules is closely related to the distribution of molecular conformations, and sampling such distributions is a fundamental challenge in physics and chemistry. Traditional methods like…

Machine Learning · Computer Science 2025-08-06 Liya Guo , Zun Wang , Chang Liu , Junzhe Li , Pipi Hu , Yi Zhu

A proof-of-concept framework for identifying molecules of unknown elemental composition and structure using experimental rotational data and probabilistic deep learning is presented. Using a minimal set of input data determined…

Chemical Physics · Physics 2020-07-01 Michael C. McCarthy , Kin Long Kelvin Lee

MINFLUX (Minimal Photon Flux) is a single-molecule imaging technique capable of resolving fluorophores at a precision of <5 nm. Interpretation of the point patterns generated by this technique presents challenges due to variable emitter…

Applications · Statistics 2026-02-24 Jack Peyton , Benjamin Davis , Emily Gribbin , Daniel Rolfe , Hannah Mitchell

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

This paper presents a probabilistic approach for online dense reconstruction using a single monocular camera moving through the environment. Compared to spatial stereo, depth estimation from motion stereo is challenging due to insufficient…

Robotics · Computer Science 2019-03-27 Yonggen Ling , Kaixuan Wang , Shaojie Shen

Single molecule imaging is one of the main target areas of X-ray free electron lasers. It relies on the possibility of orienting the large number of low counting statistics 2D diffraction patterns taken at random orientations of identical…

Atomic and Molecular Clusters · Physics 2012-11-30 Zoltan Jurek , Gyula Faigel

A method for the analysis of superresolution microscopy images is presented. This method is based on the analysis of stochastic trajectories of particles moving on the membrane of a cell with the assumption that this motion is determined by…

Optimization and Control · Mathematics 2023-11-09 Mario Annunziato , Alfio Borzì

Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…