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Single-molecule localization fluorescence microscopy constructs super-resolution images by sequential imaging and computational localization of sparsely activated fluorophores. Accurate and efficient fluorophore localization algorithms are…

Image and Video Processing · Electrical Eng. & Systems 2020-07-21 Artur Speiser , Lucas-Raphael Müller , Ulf Matti , Christopher J. Obara , Wesley R. Legant , Jonas Ries , Jakob H. Macke , Srinivas C. Turaga

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

We investigate the recovery of structures from large-area, low dose exposures that distribute the dose over many identical copies of an object. The reconstruction is done via a maximum likelihood approach that does neither require to…

Materials Science · Physics 2018-03-28 C. Kramberger , J. C. Meyer

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…

Optics · Physics 2013-09-02 Anthony Barsic , Ginni Grover , Rafael Piestun

The lensless endoscope (LE) is a promising device to acquire in vivo images at a cellular scale. The tiny size of the probe enables a deep exploration of the tissues. Lensless endoscopy with a multicore fiber (MCF) commonly uses a spatial…

In coherent imaging, speckle is statistically modeled as multiplicative noise, posing a fundamental challenge for image reconstruction. While maximum likelihood estimation (MLE) provides a principled framework for speckle mitigation, its…

Computer Vision and Pattern Recognition · Computer Science 2026-02-12 Xi Chen , Arian Maleki , Shirin Jalali

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

In order to address the issue that medical image would suffer from severe blurring caused by the lack of high-frequency details in the process of image super-resolution reconstruction, a novel medical image super-resolution method based on…

Computer Vision and Pattern Recognition · Computer Science 2019-05-14 Kewen Liu , Yuan Ma , Hongxia Xiong , Zejun Yan , Zhijun Zhou , Panpan Fang , Chaoyang Liu

We present Particle-Based Lensing (PBL), a new technique for gravitational lensing mass reconstructions of galaxy clusters. Traditionally, most methods have employed either a finite inversion or gridding to turn observational lensed galaxy…

Astrophysics · Physics 2009-11-13 Sanghamitra Deb , David M. Goldberg , Vede J. Ramdass

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

Single molecule localization microscopy (SMLM) permits the visualization of cellular structures an order of magnitude smaller than the diffraction limit of visible light, and an accurate, objective evaluation of the resolution of an SMLM…

Computational image reconstruction algorithms generally produce a single image without any measure of uncertainty or confidence. Regularized Maximum Likelihood (RML) and feed-forward deep learning approaches for inverse problems typically…

Machine Learning · Computer Science 2020-12-18 He Sun , Katherine L. Bouman

Current high-resolution imaging techniques require an intact sample that preserves spatial relationships. We here present a novel approach, "puzzle imaging," that allows imaging a spatially scrambled sample. This technique takes many…

Neurons and Cognition · Quantitative Biology 2016-02-17 Joshua I. Glaser , Bradley M. Zamft , George M. Church , Konrad P. Kording

Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the…

Applied Physics · Physics 2020-05-21 Miao-Hsuan Chien , Silvan Schmid

Particle Size Analysis (PSA) is an important process carried out in a number of industries, which can significantly influence the properties of the final product. A ubiquitous instrument for this purpose is the Optical Microscope (OM).…

Image and Video Processing · Electrical Eng. & Systems 2020-10-26 Sarvesh Patil , Chava Y P D Phani Rajanish , Naveen Margankunte

Although several image super-resolution solutions exist, they still face many challenges. CNN-based algorithms, despite the reduction in computational complexity, still need to improve their accuracy. While Transformer-based algorithms have…

Computer Vision and Pattern Recognition · Computer Science 2024-05-03 Nianzu Qiao , Lamei Di , Changyin Sun

Supervised deep learning approaches can artificially increase the resolution of microscopy images by learning a mapping between two image resolutions or modalities. However, such methods often require a large set of hard-to-get…

Image and Video Processing · Electrical Eng. & Systems 2024-11-20 Marzieh Gheisari , Auguste Genovesio

Recent advances in DNA sequencing technology and DNA storage systems have rekindled the interest in deletion channels. Multiple recent works have looked at variants of sequence reconstruction over a single and over multiple deletion…

Information Theory · Computer Science 2020-06-01 Sundara Rajan Srinivasavaradhan , Michelle Du , Suhas Diggavi , Christina Fragouli

Ultrasound localization microscopy has enabled super-resolution vascular imaging through precise localization of individual ultrasound contrast agents (microbubbles) across numerous imaging frames. However, analysis of high-density regions…

Signal Processing · Electrical Eng. & Systems 2018-12-14 Ruud J. G. van Sloun , Oren Solomon , Matthew Bruce , Zin Z. Khaing , Hessel Wijkstra , Yonina C. Eldar , Massimo Mischi

This paper proposes a probabilistic deep metric learning (PDML) framework for hyperspectral image classification, which aims to predict the category of each pixel for an image captured by hyperspectral sensors. The core problem for…

Computer Vision and Pattern Recognition · Computer Science 2022-11-16 Chengkun Wang , Wenzhao Zheng , Xian Sun , Jiwen Lu , Jie Zhou