Related papers: Phase Imaging with Computational Specificity (PICS…
Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…
Real-time monitoring of dynamic biological processes in the body is critical to understanding disease progression and treatment response. This data, for instance, can help address the lower than 50% response rates to cancer immunotherapy.…
Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…
This paper reported a general noninterferometric high-accuracy quantitative phase imaging (QPI) method for arbitrary complex valued objects. Given by a typical 4f optical configuration as the imaging system, three frames of small-window…
Resistive Pulse Sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution. As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells,…
Semantic segmentation models trained on public datasets have achieved great success in recent years. However, these models didn't consider the personalization issue of segmentation though it is important in practice. In this paper, we…
The flexible endoscope is a minimally invasive tool in clinical settings, but most of them rely on exogenous staining for diagnosis to provide qualitative information. Here, we demonstrated a flexible endoscopic microscopy (FEM) with…
We report results on unsupervised organization of cervical cells using microscopy of Pap-smear samples in brightfield (3-channel colour) as well as high resolution quantitative phase imaging modalities. A number of morphological parameters…
As a fundamental visual attribute, image complexity significantly influences both human perception and the performance of computer vision models. However, accurately assessing and quantifying image complexity remains a challenging task. (1)…
Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…
Optical phase estimation is a vital measurement primitive that is used to perform accurate measurements of various physical quantities like length, velocity and displacements. The precision of such measurements can be largely enhanced by…
Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, I$^2$PIE, a novel spectral phase control technique, was implemented in…
Studying the growth and metabolism of microbes provides critical insights into their evolutionary adaptations to harsh environments, which are essential for microbial research and biotechnology applications. In this study, we developed an…
We present a Fisher-information (FI) framework for photon-efficient fluorescence lifetime imaging microscopy (FLIM) that treats temporal sampling as a controllable design variable under a fixed photon (dose) budget. Starting from a Poisson…
In computational phase imaging with a microscope equipped with an array of light emitting diodes as illumination unit, conventional Fourier ptychographic microscopy achieves high resolution and wide-field reconstructions but is constrained…
Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of…
Understanding cell cycle dynamics is crucial for studying biological processes such as growth, development and disease progression. While fluorescent protein reporters like the Fucci system allow live monitoring of cell cycle phases, they…
Effective training of deep image segmentation models is challenging due to the need for abundant, high-quality annotations. Generating annotations is laborious and time-consuming for human experts, especially in medical image segmentation.…
Multi-spectral quantitative phase imaging (QPI) is an emerging imaging modality for wavelength dependent studies of several biological and industrial specimens. Simultaneous multi-spectral QPI is generally performed with color CCD cameras.…
Fluorescence microscopy, widely used in the study of living cells, tissues, and organisms, often faces the challenge of photodamage. This is primarily caused by the interaction between light and biochemical components during the imaging…