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Related papers: Phase Imaging with Computational Specificity (PICS…

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Fourier ptychographic microscopy (FPM) is a promising quantitative phase imaging technique that enables high-resolution, label-free imaging over a large field-of-view. Here, we present the first application of FPM for the quantitative…

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

In life sciences, fluorescent labeling techniques are used to study molecular structures and interactions of cells. However, this type of cell imaging has its own limitations, one of which is that the process of staining the cells could be…

Image and Video Processing · Electrical Eng. & Systems 2020-05-07 Eunjae Kim

We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of…

Optics · Physics 2024-10-22 Richard G. Baird , Apratim Majumder , Rajesh Menon

Purpose: Quantitative phase imaging (QPI) is a label-free technique that provides high-contrast images of tissues and cells without the use of chemicals or dyes. Accurate semantic segmentation of cells in QPI is essential for various…

Image and Video Processing · Electrical Eng. & Systems 2024-05-13 Zhuchen Shao , Mark A. Anastasio , Hua Li

Multiplexed immunofluorescence microscopy captures detailed measurements of spatially resolved, multiple biomarkers simultaneously, revealing tissue composition and cellular interactions in situ among single cells. The growing scale and…

Software Engineering · Computer Science 2025-06-03 Fang Liu , Rui He , Andrei Bombin , Ahmad B. Abdallah , Omar Eldaghar , Tommy R. Sheeley , Sam E. Ying , George Zaki

We propose a general framework for a collaborative machine learning system to assist bioscience researchers with the task of labeling specific cell identities from microscopic still or video imaging. The distinguishing features of this…

Quantitative Methods · Quantitative Biology 2019-03-25 Greg Bubnis , Steven Ban , Matthew D. DiFranco , Saul Kato

Single-pixel cameras based on the concepts of compressed sensing (CS) leverage the inherent structure of images to retrieve them with far fewer measurements and operate efficiently over a significantly broader spectral range than…

Computer Vision and Pattern Recognition · Computer Science 2016-06-17 Qiang Guo , Hongwei Chen , Yuxi Wang , Yong Guo , Peng Liu , Xiurui Zhu , Zheng Cheng , Zhenming Yu , Minghua Chen , Sigang Yang , Shizhong Xie

Photonic integrated circuits (PICs) offer ultra-broad optical bandwidths that enable unprecedented data throughputs for signal processing applications. Dynamic reconfigurability enables compensation of fabrication flaws and fluctuating…

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

Quantitative Methods · Quantitative Biology 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

Sperm cell motility and morphology observed under the bright field microscopy are the only criteria for selecting particular sperm cell during Intracytoplasmic Sperm Injection (ICSI) procedure of Assisted Reproductive Technology (ART).…

This paper presents a smartphone-based imaging system capable of quantifying the concentration of an assortment of biological/chemical assay samples. The main objective is to construct an image database which characterizes the relationship…

Image and Video Processing · Electrical Eng. & Systems 2026-04-17 Zhendong Cao , Hongji Dai , Zhida Li , Ash Parameswaran

The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

Quantitative Methods · Quantitative Biology 2024-07-18 Nathan Quiblier , Jan-Michael Rye , Pierre Leclerc , Henri Truong , Abdelkrim Hannou , Laurent Héliot , Hugues Berry

Mid-InfraRed spectroscopy is a promising label-free technique that can offer insights into morphological and pathological alterations in biological tissues at the molecular level. Owing to the development of the Fourier Transform InfraRed…

Optics · Physics 2026-04-21 Xiangyu Zhao , Yudong Tian , Jingzhu Shao , Chongzhao Wu

Interferometric methods, renowned for their reliability and precision, play a vital role in phase imaging. Interferometry typically requires high coherence and stability between the measured and the reference beam. The presence of rapid…

Phase is an intrinsic property of light, and thus a crucial parameter across numerous applications in modern optics. Various methods exist for measuring the phase of light, each presenting challenges and limitations-from the mechanical…

Fourier ptychographic microscopy (FPM) is a pivotal computational imaging technique that achieves phase and amplitude reconstruction with high resolution and wide field of view, using low numerical aperture objectives and LED array…

Quantitative phase microscopy (QPM) is a label-free technique that enables to monitor morphological changes at subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence…

Mechanical strain and stress play a major role in biological processes such as wound healing or morphogenesis. To assess this role quantitatively, fixed or live images of tissues are acquired at a cellular precision in large fields of…

Tissues and Organs · Quantitative Biology 2019-06-12 M. Durande , S. Tlili , T. Homan , B. Guirao , F. Graner , H. Delanoë-Ayari

A new numerically and experimentally tested measurement method for the local electrical light field including its phase is presented. The method is based on Self Referencing Interferograms (SRI) such as shearing interferograms. The complex…

Instrumentation and Detectors · Physics 2016-10-27 Martin Berz , Cordelia Berz